用于液相色谱-质谱联用脂质组学和代谢组学的血浆样品制备方法比较
Comparison of blood plasma sample preparation methods for combined LC-MS lipidomics and metabolomics.
作者信息
Patterson Rainey E, Ducrocq Antoine J, McDougall Danielle J, Garrett Timothy J, Yost Richard A
机构信息
Department of Chemistry, University of Florida, P.O. Box 117200, Gainesville, FL 32611, United States.
Biomedical Mass Spectrometry Laboratory, Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL 32610, United States.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Oct 1;1002:260-6. doi: 10.1016/j.jchromb.2015.08.018. Epub 2015 Aug 28.
The goal of this research was to find the most comprehensive lipid extraction of blood plasma, while also providing adequate aqueous preparation for metabolite analysis. Comparisons have been made previously of the Folch, Bligh-Dyer, and Matyash lipid extractions; furthermore, this paper provides an additional comparison of a phospholipid removal plate for analysis. This plate was used for lipid extraction rather than its intended use in lipid removal for polar analysis, and it proves to be robust for targeted lipid analysis. Folch and Matyash provided reproducible recovery over a range of lipid classes, however the Matyash aqueous layer compared well to a typical methanol preparation for polar metabolite analysis. Thus, the Matyash method is the best choice for an untargeted biphasic extraction for metabolomics and lipidomics in blood plasma.
本研究的目标是找到最全面的血浆脂质提取方法，同时为代谢物分析提供合适的水性制剂。此前已对Folch法、Bligh-Dyer法和Matyash脂质提取法进行了比较；此外，本文还对用于分析的磷脂去除板进行了额外比较。该板用于脂质提取，而非其在极性分析中去除脂质的预期用途，并且它被证明对靶向脂质分析具有稳健性。Folch法和Matyash法在一系列脂质类别中提供了可重复的回收率，然而，Matyash水层与用于极性代谢物分析的典型甲醇制剂相比效果良好。因此，Matyash法是血浆代谢组学和脂质组学非靶向双相提取的最佳选择。
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