Noé C, Lagrange B, Sayag J
C. H. U. Timone Adulte, Marseille.
Ann Dermatol Venereol. 1988;115(9):927-31.
negative test: less than 10 UI/ml; doubtful test 10-100 UI/ml; positive test greater than 100 UI/ml. HA: negative test: less than 8 dilutions; doubtful test 16-24; positive test greater than 128. The antibodies kinetics vary with the pattern of the infection (primary infection or reinfection), with the method of identification, and with the subjects. In the case of primary infection, the IgM appear since the first week following the acquisition of the infection; they reach their highest titre at the first month (variability: 15th day-3rd month); they disappear during the fourth month (variability: 2-9 months). The antibodies detected by HA after 2 ME must be reduced by at least 3 dilutions to be significant. The IgG appear later. They are detected at the beginning of the second week by IF or of the 4th month by HA, reach their highest titre at the 4th month by IF (variability: 2-9 months) or at the 9th month by HA (variability: 4th month-2nd year); they then decrease to reach lower titres within the space of 1 or 5 years. The antibodies detected in the case of reinfection are usually of higher titre with a steeper rate of increase, and are more persistent than following a primary infection.
免疫荧光法(IF):检测结果阴性:低于10国际单位/毫升;检测结果可疑:10 - 100国际单位/毫升;检测结果阳性:高于100国际单位/毫升。血凝试验(HA):检测结果阴性:低于8倍稀释;检测结果可疑:16 - 24倍稀释;检测结果阳性:高于128倍稀释。抗体动力学随感染模式(初次感染或再次感染)、鉴定方法以及个体的不同而变化。在初次感染的情况下,IgM在感染后的第一周开始出现;在第一个月达到最高滴度(变化范围:第15天 - 第3个月);在第四个月消失(变化范围:2 - 9个月)。经2 - 巯基乙醇(2 - ME)处理后,HA检测到的抗体必须至少降低3倍稀释才具有显著意义。IgG出现较晚。通过免疫荧光法在第二周初或通过血凝试验在第四个月开始检测到IgG,通过免疫荧光法在第四个月(变化范围:2 - 9个月)或通过血凝试验在第九个月(变化范围:第4个月 - 第2年)达到最高滴度;然后在1年或5年内降低至较低滴度。再次感染时检测到的抗体通常滴度更高,增加速度更快,并且比初次感染后更持久。