Wang Hui-Hui, Meng Yan-Li, Yang Zhi-Min, Wang Xiao-Xi, Xu Hui-Xing, Wang Wei-Ming
Heilongjiang Academy of Chinese Medicine Sciences Harbin 150036,China.
Zhongguo Zhong Yao Za Zhi. 2019 Dec;44(24):5473-5478. doi: 10.19540/j.cnki.cjcmm.20190716.402.
The aim of this paper was to investigate the effect of Dilong( geosaurus) on the expressions of fibrotic factors TGF-β1 and α-SMA in bleomycin-induced pulmonary fibrosis mice. The binding ability of Dilong to fibrotic factor TGF-β1 was initially detected by Biacore technology and verified by in vivo pharmacodynamics. A total of 60 SPF C57 mice were randomly divided into 6 groups. Except the blank group( injecting 0. 08 m L·kg-1 sodium chloride in the trachea),the other five groups were given bleomycin( 4 mg·kg-1) to replicate the pulmonary fibrosis model. After 14 days of drug treatment,the expressions of TGF-β1 and α-SMA were detected by Masson staining,immunohistochemistry and RT-PCR. The results of Biacore experiment showed that the extract of Dilong was well bound to TGF-β1 protein in vitro,and the binding value reached 619. 3. Compared with the model group,Masson's results showed that cellulose deposition in high-dose,medium-dose and low-dose Dilong groups decreased to varying degrees. RT-PCR results showed that different doses of Dilong could reduce protein and mRNA expressions of TGF-β1 and α-SMA to a certain extent in a dose-dependent manner. In conclusion,Dilong could delay the process of pulmonary fibrosis by binding to target protein TGF-β1 and inhibiting the expression of α-SMA.
本文旨在研究地龙对博莱霉素诱导的肺纤维化小鼠中纤维化因子转化生长因子-β1（TGF-β1）和α平滑肌肌动蛋白（α-SMA）表达的影响。首先通过Biacore技术检测地龙与纤维化因子TGF-β1的结合能力，并通过体内药效学进行验证。将60只SPF级C57小鼠随机分为6组。除空白组（气管内注射0.08 mL·kg-1氯化钠）外，其他五组给予博莱霉素（4 mg·kg-1）以复制肺纤维化模型。药物治疗14天后，通过Masson染色、免疫组织化学和逆转录-聚合酶链反应（RT-PCR）检测TGF-β1和α-SMA的表达。Biacore实验结果表明，地龙提取物在体外与TGF-β1蛋白结合良好，结合值达到619.3。与模型组相比，Masson染色结果显示，高剂量、中剂量和低剂量地龙组的纤维素沉积均有不同程度减少。RT-PCR结果表明，不同剂量的地龙可在一定程度上呈剂量依赖性降低TGF-β1和α-SMA的蛋白及mRNA表达。综上所述，地龙可通过与靶蛋白TGF-β1结合并抑制α-SMA的表达来延缓肺纤维化进程。
