Yue Na, Zhou Xue, Fei Wen-Ting, Li Ai-Min, Wang Zi-Chen, Wang Chun, Zhang Jian-Jun
School of Basic Medical Science, Beijing University of Chinese Medicine Beijing 100029, China.
New Era Health Industry(Group) Co., Ltd. Beijing 102206, China.
Zhongguo Zhong Yao Za Zhi. 2020 Mar;45(5):1004-1010. doi: 10.19540/j.cnki.cjcmm.20190916.401.
The aim of this paper was to study the effect of Lepidium meyenii(Maca) on cyclic nucleotides, neurotransmitter levels and hypothalamic-pituitary-adrenal axis and immunization of deficiency-cold and deficiency-heat syndrome rats, in order to explore the cold and hot medicinal properties of Maca. SD rats were divided into blank group, deficiency-cold syndrome group, Cinnamomi Cortex of deficiency-cold syndrome(30 g·kg(-1)) group, high and low-dose Maca groups(2.4, 1.2 g·kg(-1)), deficiency-heat syndrome group, Phellodendri Chinensis Cortex(PCC) of deficiency-heat syndrome(5 g·kg(-1)), and high and low-dose Maca groups(2.4, 1.2 g·kg(-1)). The rats were treated with intramuscular injection of hydrocortisone(20 mg·kg(-1)) or dexamethasone sodium phosphate(0.35 mg·kg(-1)) for 21 days to set up the deficiency-cold and deficiency-heat model. The levels of cAMP, cGMP, NE, DA, 5-HT, CRH, ACTH, CORT and IgM, IgG, C3, C4 were detected by radio immunoassay. Both the high-dose Maca group and the low-dose Maca group can significantly improve the overall state and body weight of rats with deficiency-cold syndrome(P<0.01, P<0.05), significantly increasing cAMP, cAMP/cGMP, NE, DA, ACTH(P<0.01, P<0.001), and significantly decreasing 5-HT(P<0.01, P<0.001). However, high-dose and low-dose Maca groups could not improve the deficiency-heat syndrome, and the levels of cAMP, cGMP, cAMP/cGMP, NE, DA, 5-HT and ACTH were not statistically significant. Maca had a significant regulatory effect on CORT, IgM, IgG and C3 content of rats with deficiency-cold and deficiency-heat syndrome(P<0.01, P<0.05, P<0.001). Maca showed the same effect with Cinnamomi Cortex in adjusting the levels of deficiency-cold rats, but in opposition to Phellodendri Chinese Cortex. This paper confirmed that Maca was slightly warm based on its effect on cyclic nucleotide levels and neuro-endocrine-immune networks by the pharmacological experimental method.
本文旨在研究玛咖对虚寒证和虚热证大鼠环核苷酸、神经递质水平、下丘脑 - 垂体 - 肾上腺轴及免疫功能的影响，以探讨玛咖的寒热药性。将SD大鼠分为空白组、虚寒证组、虚寒证肉桂组（30 g·kg⁻¹）、玛咖高、低剂量组（2.4、1.2 g·kg⁻¹）、虚热证组、虚热证黄柏组（5 g·kg⁻¹）以及玛咖高、低剂量组（2.4、1.2 g·kg⁻¹）。通过肌肉注射氢化可的松（20 mg·kg⁻¹）或地塞米松磷酸钠（0.35 mg·kg⁻¹）处理大鼠21天以建立虚寒和虚热模型。采用放射免疫分析法检测环磷酸腺苷（cAMP）、环磷酸鸟苷（cGMP）、去甲肾上腺素（NE）、多巴胺（DA）、5 - 羟色胺（5 - HT）、促肾上腺皮质激素释放激素（CRH）、促肾上腺皮质激素（ACTH）、皮质酮（CORT）以及免疫球蛋白M（IgM）、免疫球蛋白G（IgG）、补体C3、补体C4的水平。玛咖高剂量组和低剂量组均可显著改善虚寒证大鼠的整体状态和体重（P＜0.01，P＜0.05），显著升高cAMP、cAMP/cGMP、NE、DA、ACTH（P＜0.01，P＜0.001），并显著降低5 - HT（P＜0.01，P＜0.001）。然而，玛咖高、低剂量组对虚热证无改善作用，cAMP、cGMP、cAMP/cGMP、NE、DA、5 - HT及ACTH水平差异无统计学意义。玛咖对虚寒证和虚热证大鼠的CORT、IgM、IgG及C3含量有显著调节作用（P＜0.01，P＜0.05，P＜0.001）。玛咖在调节虚寒证大鼠水平方面与肉桂表现相同，但与黄柏相反。本文通过药理实验方法证实玛咖基于其对环核苷酸水平及神经 - 内分泌 - 免疫网络的影响而具有微温的特性。
