Wang Rui-Sheng, Zhang Zhen-Ling, Chen Yi-Tian, Xia Yun-Ling, Lin Xiu-Min, Zhang Jiang-Shan, Yan Meng-Zhen
Henan University of Chinese Medicine Zhengzhou 450008,China.
Zhongguo Zhong Yao Za Zhi. 2019 May;44(10):2084-2089. doi: 10.19540/j.cnki.cjcmm.20181221.004.
To reveal the transformation and attribution of drug properties in Galla Chinesis fermented Baiyaojian by studying the effect of Galla Chinesis and Baiyaojian on cold and heat syndrome rats. Euthyrox was used to induce the hyperthyrosis model,ice water stimulation was used to induce the cold syndrome model,and different concentrations of Galla Chinesis and Baiyaojian water decoction were administrated by gavage for 15 d continuously. Symptom indexes were evaluated,content of pyruvic acid( PA),ATPase activity in liver and contents of DA,T4,cAMP,5-HT,NE,17-OHCS,TRH and TSH in serum were assayed by enzyme linked immunosorbent assay and spectrophotometry. The rectal temperature,water consumption and body weight of heat syndrome rats in model group were increased,cAMP,NE,17-OHCS,TRH and PA were increased,TSH,Na-K ATPase and Ca-Mg ATPase were increased significantly( P<0. 01),while 5-HT was decreased,compared with those of the blank group( P< 0. 05),the contents of T4,DA,NE,TSH,TRH,cAMP and 17-OHCS were decreased significantly( P<0. 01),PA and Ca-Mg ATPase in WG and BG groups were decreased compared with those of the model group( P<0. 05),and the Galla Chinesis content of WG group was lower than that of BG group,while the contents of 5-HT in WG and BG groups were increased,and the Galla Chinesis content of WG group was higher than that of BG group,with no significant difference of viscera index between heat syndrome rats in blank group,model group and drug groups. The rectal temperature,water consumption and body weight of cold syndrome rats in model group were decreased,DA,T4,cAMP,NE,17-OHCS,TRH,TSH,PA,Na-K ATPase and Ca-Mg ATPase of rats in model group were decreased,whereas 5-HT was increased compared with those of the blank group( P<0. 05),the indexes of heart,lung and kidney were significantly higher than those in the blank group( P<0. 05). Both Galla Chinesis and Baiyaojian can significantly alleviate the symptoms of heat syndrome rats caused by levothyroxine sodium. Galla Chinesis has a better effect than Baiyaojian,but cannot alleviate the symptoms of cold syndrome caused by ice water stimulation,suggestting that the decoction of Galla Chinesis and Baiyaojian are both cold,but Galla Chinesis is colder than Baiyaojian. Cold property in Galla Chinesis fermented Baiyaojian can be relieved. In clinical application,the property of " slight cold" is more accurate than " neutral property" for Baiyaojian.
通过研究五倍子和白药煎剂对寒证和热证大鼠的影响，揭示五倍子发酵白药煎剂中药物性质的转变及归属。用优甲乐诱导甲状腺功能亢进模型，用冰水刺激诱导寒证模型，连续15天灌胃给予不同浓度的五倍子和白药煎剂。评估症状指标，采用酶联免疫吸附测定法和分光光度法测定丙酮酸（PA）含量、肝脏ATP酶活性以及血清中多巴胺（DA）、甲状腺素（T4）、环磷酸腺苷（cAMP）、5-羟色胺（5-HT）、去甲肾上腺素（NE）、17-羟皮质类固醇（17-OHCS）、促甲状腺激素释放激素（TRH）和促甲状腺激素（TSH）的含量。与空白组相比，模型组热证大鼠的直肠温度、饮水量和体重增加，cAMP、NE、17-OHCS、TRH和PA升高，TSH、钠钾ATP酶和钙镁ATP酶显著升高（P<0.01），而5-HT降低（P<0.05），WG组和BG组的T4、DA、NE、TSH、TRH、cAMP和17-OHCS含量与模型组相比显著降低（P<0.01），WG组和BG组的PA和钙镁ATP酶与模型组相比降低（P<0.05），WG组五倍子含量低于BG组，而WG组和BG组5-HT含量升高，WG组五倍子含量高于BG组，空白组、模型组和药物组热证大鼠的脏器指数无显著差异。模型组寒证大鼠的直肠温度、饮水量和体重降低，模型组大鼠的DA、T4、cAMP、NE、17-OHCS、TRH、TSH、PA、钠钾ATP酶和钙镁ATP酶降低，而5-HT与空白组相比升高（P<0.05），心、肺、肾指标显著高于空白组（P<0.05）。五倍子和白药煎剂均能显著缓解左甲状腺素钠所致热证大鼠的症状。五倍子的效果优于白药煎剂，但不能缓解冰水刺激所致寒证的症状，提示五倍子和白药煎剂均属寒性，但五倍子寒性更强。五倍子发酵白药煎剂的寒性可减轻。在临床应用中，白药煎剂用“微寒”属性比“平性”更准确。
