Shi Xiao-Hong, Yang Ri-Zhao, Zhao Li-Jie, Hong Yan-Long, Feng Yi, Wang You-Jie
Engineering Research Center of Modern Preparation Technology of Traditional Chinese Medicine under Ministry of Education,Innovation Research Institute of TCM, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
Shanghai Innovation Center of Traditional Chinese Medicine Health Service, Shanghai University of Traditional Chinese Medicine Shanghai 201203, China.
Zhongguo Zhong Yao Za Zhi. 2020 Feb;45(4):846-853. doi: 10.19540/j.cnki.cjcmm.20191217.306.
To explore the relationship between the variations of the physiochemical properties of traditional Chinese medicine(TCM) decoction before or after precipitation in alcohol and the wall stickiness in spray drying. In this study, widely used TCMs in clinic were selected to determine the physiochemical properties of TCM decoction before or after precipitation in alcohol separately.Afterwards, the principle component analysis(PCA),Hierarchical cluster analysis(HCA),and orthogonal partial least squares-discriminate analysis(OPLS-DA) were used to evaluate the relationship between the variations of those liquid before or after precipitation in alcohol and hot-melt stickiness in spray drying.Three types of statistical analysis methods all indicated that ethanol precipitation affected physiochemical properties of TCM decoction, and the variations of physical properties showed significant association with hot-melt stickiness in spray drying.The results of PCA-X and HCA suggested that the dynamic surface tension(DST) was impacted most by the alcohol deposition treatment,at the same time,the other 5 physiochemical properties were also affected.OPLS-DA verified that PCA-X and HCA results, and revealed that DST,equilibrium surface tension(EST) and pH were significantly affected by alcohol deposition treatment,and the order of the affecting factors from high to low was DST,EST and pH.Therefore,the downward trend of DST and pH were the important factors that directly affected the hot-melt stickiness of TCM after precipitation in alcohol,which would be probably caused by losing macromolecules alcoholic insoluble components and increasing relative proportions of organic acid and small molecule sugar.
探讨中药水煎液乙醇沉淀前后理化性质的变化与喷雾干燥过程中壁黏附性之间的关系。本研究选取临床常用的中药，分别测定其水煎液乙醇沉淀前后的理化性质。之后，采用主成分分析（PCA）、层次聚类分析（HCA）和正交偏最小二乘法判别分析（OPLS-DA），评价乙醇沉淀前后液体性质变化与喷雾干燥热熔黏附性之间的关系。三种统计分析方法均表明，乙醇沉淀影响中药水煎液的理化性质，其物理性质的变化与喷雾干燥热熔黏附性显著相关。PCA-X和HCA结果表明，乙醇沉淀处理对动态表面张力（DST）影响最大，同时其他5种理化性质也受到影响。OPLS-DA验证了PCA-X和HCA的结果，并揭示乙醇沉淀处理对DST、平衡表面张力（EST）和pH有显著影响，影响因素由高到低依次为DST、EST和pH。因此，DST和pH的下降趋势是直接影响乙醇沉淀后中药热熔黏附性的重要因素，这可能是由于大分子醇不溶性成分流失以及有机酸和小分子糖相对比例增加所致。
