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[乌天麻病害的调查、分析与鉴定]

[Investigation, analysis and identification of disease of Gastrodia elata f. glauca].

作者信息

Zhang Jin-Qiang, Tang Xin, Xiao Cheng-Hong, Xu Jiao, Yuan Qing-Song, Wang Xiao, Liu Da-Hui, Zhang Guang-Wen, Liu Fu-Ming, Jiang Wei-Ke, Zhou Tao

机构信息

Guizhou University of Traditional Chinese Medicine Guiyang 550025, China.

Shandong Analysis and Test Center Jinan 250014, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2020 Feb;45(3):478-484. doi: 10.19540/j.cnki.cjcmm.20191204.108.

Abstract

Fungal disease is an important factor restricting the healthy development of Gastrodia elata industry. The control of fungal disease in G. elata is an important issue in production. This paper makes a detailed investigation on the current situation of G. elata disease in China through statistics on the failure rate, rotten pit rate and occurrence rate of G. elata disease in the main producing areas of China. It was found that G. elata disease was mainly infected from the top bud and junction, causing the occurrence rate of disease was 6%-17%, and the yield decreased by 10%-30%. The 23 dominant fungi were isolated from 18 typical G. elata disease samples. Through identification of colony morphology, mycelium morphology, spore morphology and genetic characteristics, they were finally identified as 13 species, belonging to 7 families and 7 genera. Trichoderma harzianum, Ilyonectria sp. and Ilyonectria destructans are the most frequently separated. Their isolation frequency were 22.22%,16.67%,16.67% respectively. Ilyonectria sp. and I. destructans were the first time isolated from G. elata disease samples. They may be the main pathogens causing soil-borne diseases of G. elata. T. harzianum has certain potential as Gastrodia biocontrol bacteria. This study can provide a theoretical basis for the research and development of control technology of Gastrodia fungi disease.

摘要

真菌病害是制约天麻产业健康发展的重要因素。天麻真菌病害的防治是生产中的重要问题。本文通过对我国天麻主产区天麻病害的发病率、烂窖率及发病株率进行统计,详细调查了我国天麻病害现状。结果发现,天麻病害主要从顶芽及接口处侵染,发病率为6%-17%,产量下降10%-30%。从18份典型天麻病害样本中分离出23种优势真菌。通过对菌落形态、菌丝形态、孢子形态及遗传特性的鉴定,最终确定为13个种,分属于7科7属。哈茨木霉、间座壳菌和毁灭间座壳菌分离频率最高,分离频率分别为22.22%、16.67%、16.67%。间座壳菌和毁灭间座壳菌首次从天麻病害样本中分离得到,可能是引起天麻土传病害的主要病原菌。哈茨木霉作为天麻生防菌具有一定潜力。本研究可为天麻真菌病害防治技术的研发提供理论依据。

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