Vocational School of Health Service, Bilecik Seyh Edebali University, Bilecik, Turkey.
Comb Chem High Throughput Screen. 2020;23(9):939-944. doi: 10.2174/1386207323666200402080557.
Propolis is a resinous mixture collected by honeybees from tree buds and exudates of the plants. Propolis contains aromatic acids, diterpenic acids and phenolic compounds and these components are responsible for its antitumor, anticancer, antiviral and antifungal effects. Propolis can be extracted and the solubility of propolis differs depending on the solvent used in the process of extraction. Solvents used for propolis extraction have a great impact on the propolis extract and should be nontoxic.
In this study, raw propolis was extracted by peppermint and clove volatile oils.
Chemical composition of extracts was determined by using GC-MS equipment. Total phenolic content and antioxidant activity of the extracts were measured. α-amylase inhibitory activity of the extracts was carried out as well.
The findings of the present study showed that clove volatile oil is more effective in the extraction of propolis than peppermint volatile oil. The total phenolic content of these extracts was determined as 175.12 and 40.80 mg GAE/mL for clove and peppermint oil propolis extracts, respectively. All extracts contained the same phenolic compounds but the quantity was less in volatile oil extract than in ethanol extract. Both of these extracts showed better α-amylase ınhibitory activity than a reference inhibitor, acarbose.
It could be concluded that propolis extract obtained by using volatile oils could be used as a complementary agent in the treatment of type 2 diabetes mellitus.
蜂胶是蜜蜂从树芽和植物渗出物中采集的树脂状混合物。蜂胶含有芳香酸、二萜酸和酚类化合物，这些成分是其具有抗肿瘤、抗癌、抗病毒和抗真菌作用的原因。蜂胶可以被提取出来，其溶解度取决于提取过程中使用的溶剂。用于提取蜂胶的溶剂对蜂胶提取物有很大的影响，应该是无毒的。
本研究采用薄荷油和丁香油提取原蜂胶。
采用 GC-MS 设备测定提取物的化学成分。测定提取物的总酚含量和抗氧化活性。并进行提取物的α-淀粉酶抑制活性实验。
本研究结果表明，丁香油比薄荷油更有效地提取蜂胶。这些提取物的总酚含量分别为 175.12 和 40.80mg GAE/mL。两种提取物均含有相同的酚类化合物，但在挥发油提取物中的含量低于乙醇提取物。这两种提取物的α-淀粉酶抑制活性均优于对照抑制剂阿卡波糖。
可以得出结论，使用挥发油提取的蜂胶提取物可作为治疗 2 型糖尿病的辅助药物。
