丁香（Syzygium aromaticum (L.) Merr. & Perry）芽中富含多酚的提取物对与2型糖尿病相关的碳水化合物水解酶的体外抑制活性以及对大鼠胰腺中铁离子（Fe(2+)）诱导的脂质过氧化的影响
In vitro inhibition activity of polyphenol-rich extracts from Syzygium aromaticum (L.) Merr. & Perry (Clove) buds against carbohydrate hydrolyzing enzymes linked to type 2 diabetes and Fe(2+)-induced lipid peroxidation in rat pancreas.
作者信息
Adefegha Stephen Adeniyi, Oboh Ganiyu
机构信息
Department of Biochemistry, Federal University of Technology, Akure, P.M.B. 704, Akure-340001, Nigeria.
出版信息
Asian Pac J Trop Biomed. 2012 Oct;2(10):774-81. doi: 10.1016/S2221-1691(12)60228-7.
OBJECTIVE
To investigate and compare the inhibitory properties of free and bound phenolic extracts of clove bud against carbohydrate hydrolyzing enzymes (alpha-amylase & alpha-glucosidase) and Fe(2+)-induced lipid peroxidation in rat pancreas in vitro.
METHODS
The free phenolics were extracted with 80% (v/v) acetone, while bound phenolics were extracted from the alkaline and acid hydrolyzed residue with ethyl acetate. Then, the interaction of the extracts with alpha-amylase and alpha-glucosidase was subsequently assessed. Thereafter, the total phenolic contents and antioxidant activities of the extracts were determined.
RESULTS
The result revealed that both extracts inhibited alpha-amylase and alpha-glucosidase in a dose-dependent manner. However, the alpha-glucosidase inhibitory activity of the extracts were significantly (P<0.05) higher than their alpha-amylase inhibitory activity. The free phenolics (31.67 mg/g) and flavonoid (17.28 mg/g) contents were significantly (P<0.05) higher than bound phenolic (23.52 mg/g) and flavonoid (13.70 mg/g) contents. Both extracts also exhibited high antioxidant activities as typified by their high reducing power, 1,1 diphenyl-2- picrylhydrazyl (DPPH) and 2, 2-azinobis-3-ethylbenzo-thiazoline-6-sulfonate (ABTS) radical scavenging abilities, as well as inhibition of Fe(2+)-induced lipid peroxidation in rat pancreas in vitro.
CONCLUSIONS
This study provides a biochemical rationale by which clove elicits therapeutic effect on type 2 diabetes.
目的
研究并比较丁香花蕾中游离酚类提取物和结合酚类提取物对碳水化合物水解酶（α-淀粉酶和α-葡萄糖苷酶）的抑制特性，以及在体外对大鼠胰腺铁（Ⅱ）诱导的脂质过氧化的抑制特性。
方法
用80%（v/v）丙酮提取游离酚类，而结合酚类则从碱水解和酸水解残渣中用乙酸乙酯提取。随后评估提取物与α-淀粉酶和α-葡萄糖苷酶的相互作用。此后，测定提取物的总酚含量和抗氧化活性。
结果
结果显示，两种提取物均以剂量依赖性方式抑制α-淀粉酶和α-葡萄糖苷酶。然而，提取物的α-葡萄糖苷酶抑制活性显著（P<0.05）高于其α-淀粉酶抑制活性。游离酚类（31.67 mg/g）和黄酮类（17.28 mg/g）含量显著（P<0.05）高于结合酚类（23.52 mg/g）和黄酮类（13.70 mg/g）含量。两种提取物还表现出较高的抗氧化活性，其特征在于具有较高的还原能力、1,1-二苯基-2-苦基肼（DPPH）和2,2-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除能力，以及在体外对大鼠胰腺铁（Ⅱ）诱导的脂质过氧化的抑制作用。
结论
本研究为丁香对2型糖尿病产生治疗作用提供了生化依据。
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