Coluccini María Laura
Universidad Nacional de C´órdoba. Facultad de Ciencias Médicas. Instituto de Virología Dr. José María Vanella; Argentina.
Rev Fac Cien Med Univ Nac Cordoba. 2020 Mar 18;77(1):15-18. doi: 10.31053/1853.0605.v77.n1.24070.
The Norovirus (NoV) constitute a genus within the viral family Caliciviridae, being the main cause of outbreaks of food origin among humans. Fresh vegetables are susceptible to being contaminated with these pathogens during their cultivation, harvest, transport, processing and handling. So it was intended to determine the frequency of detection of NoV in plant samples of leaves of the City of Córdoba, and adapt a method of viral concentration with polyethylene glycol for the recovery of viral particles from the surface of vegetables and characterize the genogroups of NoV detected.
19 samples of leafy vegetables were taken between June and December 2012. A viral concentration technique previously validated in the laboratory was applied (elution and precipitation with polyethylene glycol). The viral RNA was extracted to the concentrates of the samples using Trizol and precipitation with isopropyl alcohol. The nucleic acid was amplified by Rt-PCR with specific primers to identify genogroups I (GI) and II (GII). The products of the amplification were revealed by polyacrylamide gel electrophoresis and silver staining.
We found 57.89% positive samples. Ten of the detected strains belonged to genogroup I (GI) and one to genogroup II (GII). They were identified throughout the study period, particularly during the months of August, September and November.
These pathogens were detected with a prevalence of 57.89%. The strains belonged mainly to the GI, representing a potential risk for the population.
诺如病毒（NoV）属于杯状病毒科中的一个属，是人类食源性疾病暴发的主要原因。新鲜蔬菜在种植、收获、运输、加工和处理过程中容易受到这些病原体的污染。因此，本研究旨在确定科尔多瓦市叶片植物样本中诺如病毒的检出频率，采用聚乙二醇病毒浓缩方法从蔬菜表面回收病毒颗粒，并对检出的诺如病毒基因群进行鉴定。
于2012年6月至12月采集了19份叶菜类蔬菜样本。应用实验室先前验证的病毒浓缩技术（用聚乙二醇洗脱和沉淀）。使用Trizol和异丙醇沉淀从样本浓缩物中提取病毒RNA。通过逆转录聚合酶链反应（Rt-PCR）用特异性引物扩增核酸，以鉴定基因群I（GI）和基因群II（GII）。扩增产物通过聚丙烯酰胺凝胶电泳和银染进行显示。
我们发现57.89%的样本呈阳性。检出的菌株中有10株属于基因群I（GI），1株属于基因群II（GII）。在整个研究期间均有检出，尤其是在8月、9月和11月。
这些病原体的检出率为57.89%。菌株主要属于GI，对人群构成潜在风险。
