School of Materials Engineering, Shanghai University of Engineering Science, Shanghai, 201620, China.
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao, 266109, China.
Sci Rep. 2020 Apr 1;10(1):5752. doi: 10.1038/s41598-020-62495-w.
Surface Enhanced Raman Scattering (SERS) has been widely applied in many research fields such as biological detection and chemical analysis. However, for the common Au nanoparticles, it's too hard to guarante the three aspects: the great enhanced effect, the controllable aggregation and the uniformity of nanoparticles, the environmental friendliness and biocompatibility of nanoparticles. In this paper, phytate acid (IP6)-coated Au nanoparticles (IP6@Au NPs) are more stable and have a higher enhancement factor than Au nanoparticles. In order to achieve the uniformity of the spherical IP6-coated@Au nanoparticles (IP6@Au NPs), IP6 was used as a soft template. In the presence of IP6, IP6@Ag nanoparticles were first synthesized by reducing AgNO with trisodium citrate, then IP6@Au NPs were obtained by reducing HAuCl with Ag nanoparticles. The IP6@Au NPs exhibit excellent Raman signal enhancement by using p-aminothiophenol (4-ATP) as the probe molecules. The effects of Fe on the performance of IP6@Au NPs SERS substrates were also studied. The results show that SERS has the best enhancement effect when adding proper amount of Fe (0.56 PPM), and the limit of detection was 10 M 4-ATP.
表面增强拉曼散射 (SERS) 已广泛应用于生物检测和化学分析等多个研究领域。然而，对于普通的金纳米粒子而言，很难同时保证三个方面：强增强效应、可控聚集以及纳米粒子的均匀性、纳米粒子的环境友好性和生物相容性。在本文中，植酸 (IP6) 包覆的金纳米粒子 (IP6@Au NPs) 比金纳米粒子更稳定，增强因子更高。为了实现球形 IP6 包覆的@Au 纳米粒子 (IP6@Au NPs) 的均匀性，使用植酸 (IP6) 作为软模板。在 IP6 的存在下，先用柠檬酸三钠还原 AgNO3 合成 IP6@Ag 纳米粒子，然后用 Ag 纳米粒子还原 HAuCl 得到 IP6@Au NPs。以对氨基苯硫酚 (4-ATP) 作为探针分子，IP6@Au NPs 表现出优异的拉曼信号增强。还研究了 Fe 对 IP6@Au NPs SERS 基底性能的影响。结果表明，当添加适量的 Fe (0.56 PPM) 时，SERS 具有最佳的增强效果，4-ATP 的检出限为 10 M。
