Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
Graduate Institute of Clinical Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
Sci Rep. 2020 Apr 1;10(1):5771. doi: 10.1038/s41598-020-62733-1.
Household air pollution has adverse effects on cardiovascular health. One of the major sources of household air pollutants is the combustion of cooking oils during cooking. Trans, trans-2,4-decadienal (tt-DDE) is a type of dienaldehyde that is present in a wide range of food and food products. It is a byproduct of the peroxidation of linoleic acid following the heating of oil during cooking. The mechanisms of the associations between household air pollution and cardiac arrhythmias are currently unclear. The purpose of this study was to determine effects of tt-DDE on the ion currents in H9c2 cells. The I and I in H9c2 cells treated with and without tt-DDE were measured using the whole-cell patch clamp method. Expressions of Kv2.1 and Cav1.2 in H9c2 cells treated with and without tt-DDE were measured by western blot analysis. After the H9c2 cells had been exposed to tt-DDE, the I and I were significantly decreased. The expression of Kv2.1, unlike that of Cav1.2, was also significantly decreased in these cells. These changes in I and I that were induced by tt-DDE may help to explain the association between cardiac arrhythmogenesis and cooking-oil fumes.
家庭空气污染对心血管健康有不良影响。家庭空气污染物的主要来源之一是烹饪过程中食用油的燃烧。反式，反式-2,4-癸二烯醛（tt-DDE）是一种存在于广泛的食物和食品中的二烯醛。它是食用油在烹饪过程中加热时，亚油酸过氧化产生的副产物。家庭空气污染与心律失常之间关联的机制目前尚不清楚。本研究旨在确定 tt-DDE 对 H9c2 细胞离子电流的影响。采用全细胞膜片钳法测量经 tt-DDE 处理和未经 tt-DDE 处理的 H9c2 细胞中的 I 和 I。通过 Western blot 分析测量经 tt-DDE 处理和未经 tt-DDE 处理的 H9c2 细胞中 Kv2.1 和 Cav1.2 的表达。H9c2 细胞暴露于 tt-DDE 后，I 和 I 明显降低。与 Cav1.2 不同，Kv2.1 的表达也明显降低。tt-DDE 诱导的 I 和 I 的这些变化可能有助于解释烹饪油烟与心律失常发生之间的关联。
