Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, Murcia E-30100, Spain.
Analyst. 2020 May 21;145(10):3645-3655. doi: 10.1039/c9an02523e. Epub 2020 Apr 2.
In this work, we present a new catechol amperometric biosensor fabricated on the basis of naturally available enzymes in common mushrooms. The biosensor response mechanism comprises the reduction of the quinone exclusively produced in the oxidation of the catechol present in the sample, which is catalyzed by tyrosinase enzyme. The new catechol biosensor has demonstrated excellent analytical performance at increasing catechol concentrations in the sample solution, which includes superior reproducibility for several electrodes and long-term stability. On top of that, the biosensing element used in the fabrication is a sustainable material, of low-cost and presents an excellent lifetime of years. Whether the catechol biosensor is operating in the presence of a compound influencing the reactions underlying the amperometric response (such as ascorbic, benzoic, gallic and kojic acids), this serves as an analytical platform to detect these compounds in real samples. Particularly, we introduce herein for the first time different treatments to process the current signal of the biosensor pursuing the linearity needed for the analytical application in real samples. In this sense, the catechol biosensor has been successfully applied to the detection of benzoic, gallic and kojic acids in juices, teas and cosmetic products, respectively.
在这项工作中，我们提出了一种基于常见蘑菇中天然存在的酶的新型儿茶酚安培生物传感器。生物传感器的响应机制包括在样品中存在的儿茶酚的氧化过程中专门产生的醌的还原，该过程由酪氨酸酶催化。新型儿茶酚生物传感器在增加样品溶液中儿茶酚浓度时表现出出色的分析性能，包括对多个电极的出色重现性和长期稳定性。除此之外，用于制造的生物传感元件是一种可持续材料，成本低，具有数年的出色寿命。无论儿茶酚生物传感器是否在影响安培响应基础反应的化合物（如抗坏血酸、苯甲酸、没食子酸和曲酸）存在下运行，它都可以作为一种分析平台来检测实际样品中的这些化合物。特别是，我们首次引入了不同的处理方法来处理生物传感器的电流信号，以实现实际样品分析应用所需的线性度。在这种意义上，儿茶酚生物传感器已成功应用于果汁、茶和化妆品中苯甲酸、没食子酸和曲酸的检测。
