一种基于金纳米颗粒、磷酸二己酯和酪氨酸酶的生物传感器，用于测定天然水中的邻苯二酚。
A biosensor based on gold nanoparticles, dihexadecylphosphate, and tyrosinase for the determination of catechol in natural water.
作者信息
Campanhã Vicentini Fernando, Garcia Lívia L C, Figueiredo-Filho Luiz C S, Janegitz Bruno C, Fatibello-Filho Orlando
机构信息
Department of Chemistry, Federal University of São Carlos, Rod. Washington Luís km 235, P.O. Box 676, 13560-970 São Carlos, SP, Brazil; Center of Nature Sciences, Federal University of São Carlos, Rod. Lauri Simões de Barros km 12, 18290-000 Buri, SP, Brazil.
Department of Chemistry, Federal University of São Carlos, Rod. Washington Luís km 235, P.O. Box 676, 13560-970 São Carlos, SP, Brazil.
出版信息
Enzyme Microb Technol. 2016 Mar;84:17-23. doi: 10.1016/j.enzmictec.2015.12.004. Epub 2015 Dec 12.
In this work, a biosensor using a glassy carbon electrode modified with gold nanoparticles (AuNPs) and tyrosinase (Tyr) within a dihexadecylphosphate film is proposed. Cystamine and glutaraldehyde crosslinking agents were used as a support for Tyr immobilization. The proposed biosensor was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and cyclic voltammetry in the presence of catechol. The determination of catechol was carried out by amperometry and presented a linear concentration range from 2.5×10(-6) to 9.5×10(-5)molL(-1) with a detection limit of 1.7×10(-7)molL(-1). The developed biosensor showed good repeatability and stability. Moreover, this novel amperometric method was successfully applied in the determination of catechol in natural water samples. The results were in agreement with a 95% confidence level for those obtained using the official spectrophotometric method.
在本工作中，提出了一种生物传感器，该传感器采用修饰有金纳米颗粒（AuNPs）和酪氨酸酶（Tyr）的玻碳电极，且酪氨酸酶位于磷酸二己酯膜内。胱胺和戊二醛交联剂用作酪氨酸酶固定化的载体。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）以及在儿茶酚存在下的循环伏安法对所提出的生物传感器进行了表征。采用安培法测定儿茶酚，其线性浓度范围为2.5×10⁻⁶至9.5×10⁻⁵mol·L⁻¹，检测限为1.7×10⁻⁷mol·L⁻¹。所开发的生物传感器具有良好的重复性和稳定性。此外，这种新型安培法成功应用于天然水样中儿茶酚的测定。结果与使用官方分光光度法获得的结果在95%置信水平上一致。
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