凝血酶和组胺刺激下人脐静脉内皮细胞中肌醇磷酸的产生及钙离子动员
Inositol phosphate production and Ca2+ mobilization in human umbilical-vein endothelial cells stimulated by thrombin and histamine.
作者信息
Pollock W K, Wreggett K A, Irvine R F
机构信息
Department of Biochemistry, AFRC Institute of Animal Physiology, Babraham, Cambridge, U.K.
出版信息
Biochem J. 1988 Dec 1;256(2):371-6. doi: 10.1042/bj2560371.
Abstract
Human umbilical-vein endothelial cells (HUVECs) were cultured, and their inositol phosphate formation and Ca2+ mobilization in response to thrombin and histamine were studied. Evidence from measurement of intracellular Ca2+ in the absence of extracellular Ca2+ established that the two agonists were both acting on a single cell population, and suggested that a Ca2+-influx component was stimulated which was dependent on receptor-occupancy. After 30 s of stimulation in the presence of 10 mM-LiCl, the effects of 20 microM-histamine and 1 unit of thrombin/ml on formation of inositol phosphates were additive, but at 5 min they were not. HUVECs labelled with myo-[3H]inositol for 72 h synthesized radiolabelled inositol pentakis- and hexakis-phosphate. The predominant isomers of inositol mono-, bis- and tris-phosphates whose formation was stimulated were the 4-phosphate, the 1,4-bisphosphate and the 1,3,4-trisphosphate.
摘要
培养人脐静脉内皮细胞（HUVECs），并研究其对凝血酶和组胺的肌醇磷酸形成及Ca2+动员情况。在无细胞外Ca2+条件下测量细胞内Ca2+的证据表明，这两种激动剂均作用于单个细胞群体，并提示刺激了一种依赖于受体占据的Ca2+内流成分。在10 mM-LiCl存在下刺激30 s后，20 microM-组胺和1单位凝血酶/毫升对肌醇磷酸形成的作用是相加的，但在5分钟时则不是。用肌醇-[3H]标记72小时的HUVECs合成了放射性标记的肌醇五磷酸和六磷酸。受刺激形成的肌醇单磷酸、双磷酸和三磷酸的主要异构体分别是4-磷酸、1,4-双磷酸和1,3,4-三磷酸。
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