College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
Key Laboratory for Animal-Derived Food Safety of Henan Province, Zhengzhou, China.
Microbiologyopen. 2020 Jul;9(7):e1036. doi: 10.1002/mbo3.1036. Epub 2020 Apr 1.
Porcine deltacoronavirus (PDCoV) is a novel enteropathogenic coronavirus that causes watery diarrhea in piglets. Little is known regarding the alteration of the gut microbiota in PDCoV-induced diarrhea piglets. In this study, 5-day-old piglets were experimentally infected with PDCoV strain CH-01, and all piglets developed typical clinical disease, characterized by acute and severe watery diarrhea. Histologic lesions were limited to the villous epithelium of the duodenum and ileum. Gut microbiota profiles in the colon and feces of piglets inoculated with PDCoV were investigated using 16S rRNA sequencing. The results showed that PDCoV infection reduced bacterial diversity and significantly altered the composition of the microbiota from the phylum to the genus level in the colon and feces of piglets. Firmicutes (phylum), Lactobacillaceae (family), and Lactobacillus (genus) were significantly increased (p < .01), while the abundance of Bacteroidetes (phylum) was markedly reduced in the colon and feces of the PDCoV-infected piglets (p < .01) when compared to those of the healthy piglets. Furthermore, microbial function prediction indicated that the changes in the intestinal flora also affected the nucleotide transport and metabolism, defense, translation, and transcription function of the intestinal microbiota. The current study provides new insight into the pathology and physiology of PDCoV.
猪德尔塔冠状病毒（PDCoV）是一种新型的肠致病性冠状病毒，可导致仔猪水样腹泻。目前对于 PDCoV 感染引起的腹泻仔猪肠道微生物群的改变知之甚少。在本研究中，将 5 日龄仔猪用 PDCoV 株 CH-01 进行人工感染，所有仔猪均出现典型的临床疾病，表现为急性和严重的水样腹泻。组织学病变仅限于十二指肠和回肠的绒毛上皮。采用 16S rRNA 测序技术研究了接种 PDCoV 的仔猪结肠和粪便中的肠道微生物群谱。结果表明，PDCoV 感染降低了细菌多样性，并显著改变了仔猪结肠和粪便中微生物群从门到属水平的组成。厚壁菌门（门）、乳杆菌科（科）和乳酸杆菌属（属）显著增加（p <.01），而拟杆菌门（门）的丰度在 PDCoV 感染仔猪的结肠和粪便中明显降低（p <.01）与健康仔猪相比。此外，微生物功能预测表明，肠道菌群的变化也影响了肠道微生物群的核苷酸转运和代谢、防御、翻译和转录功能。本研究为 PDCoV 的病理学和生理学提供了新的见解。
