Molecular Entomology Laboratory, Department of Plant Protection, Faculty of Agriculture, Ankara University, 06110, Diskapi, Ankara, Turkey.
Department of Entomology, University of Sargodha, Sargodha, 40100, Pakistan.
Curr Microbiol. 2020 Aug;77(8):1525-1531. doi: 10.1007/s00284-020-01969-6. Epub 2020 Apr 2.
As an important insect vector, Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae) transmits the pathogen 'Candidatus Liberibacter asiaticus' (CLas) that is associated with citrus greening also known as Huanglongbing (HLB) disease. The bacterial endosymbionts have a potential role in shaping the host range of insect herbivores and their performance on different host plants, which might affect the endosymbiont distribution in insect populations. Here, we detected and characterized Pantoea endosymbiont in nymph and adult ACP specimens collected from Citrus reticulata Blanco and Cordia myxa L. plants. The phylogenetic tree constructed using endosymbiotic bacteria 16S ribosomal RNA sequences indicated that Pantoea sp. was closely related to Mixta calida, sharing about 98% identity and was grouped with other Mixta and Pantoea endosymbionts. Our findings showed 100% and 92.3% infection of Pantoea in adults while 61.5% and 90% infection of Pantoea in nymphs collected from C. reticulata and C. myxa plants, respectively. Understanding the interaction of endosymbiotic bacteria with ACP associated with host plants could be useful for developing an effective management strategy for both ACP and HLB disease.
作为一种重要的昆虫媒介，亚洲柑橘木虱（ACP），Diaphorina citri Kuwayama（半翅目：丽木虱科）传播与柑橘黄龙病（HLB）相关的病原体“Candidatus Liberibacter asiaticus”（CLas）。细菌内共生体在塑造昆虫食草动物的宿主范围及其在不同宿主植物上的表现方面具有潜在作用，这可能会影响昆虫种群中内共生体的分布。在这里，我们从柑橘属和蓝果树属植物上采集的柑橘木虱若虫和成虫标本中检测并鉴定了 Pantoea 共生菌。使用内共生细菌 16S 核糖体 RNA 序列构建的系统发育树表明，Pantoea sp.与 Mixta calida 密切相关，具有约 98%的同一性，并与其他 Mixta 和 Pantoea 内共生菌归为一组。我们的研究结果表明，从柑橘属和蓝果树属植物上采集的成虫中 Pantoea 的感染率分别为 100%和 92.3%，而若虫中 Pantoea 的感染率分别为 61.5%和 90%。了解内共生细菌与与宿主植物相关的 ACP 的相互作用，可能有助于制定针对 ACP 和 HLB 疾病的有效管理策略。
