State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519000, PR China, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China; Department of Biotechnology, Dayananda Sagar College of Engineering (Affiliated to Visvesvaraya Technological University, Belagavi), Bangalore 560111, Karnataka, India.
State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519000, PR China, School of Life Sciences, Sun Yat-Sen University, Guangzhou, PR China.
Genomics. 2020 Jul;112(4):2842-2844. doi: 10.1016/j.ygeno.2020.03.023. Epub 2020 Mar 30.
Strain DRQ-2 (type strain of Nonomuraea indica) is worthy for genome sequencing, due to its ability to produce a wide variety of industrially important enzymes such as amylase, asparaginase, cellulase, gelatinase, glutaminase, and protease. Genome sequencing and comparison of strain DRQ-2 is described in the present work. The genome size was estimated to be 8,288,417 (bp) that consisted of 59 contigs. The G + C content of the genome was 72.4%. A total of 7730 genes were predicted with two rRNAs and 64 tRNAs. The genome analysis of the strain DRQ-2 showed the presence of a wide range of secondary metabolite gene clusters. Pan-Genomes Analysis Pipeline (PGAP) indicated that strain DRQ-2 had large numbers of unique genes. The majority of N. indica DRQ-2 genes encode for hypothetical proteins, indicating the functions of these ortholog clusters were still remain to be determined.
DRQ-2 菌株（野野村菌的模式菌株）因其能够产生多种工业上重要的酶（如淀粉酶、天冬酰胺酶、纤维素酶、明胶酶、谷氨酰胺酶和蛋白酶）而适合进行基因组测序。本工作描述了 DRQ-2 菌株的基因组测序和比较。基因组大小估计为 8,288,417（bp），由 59 个 contigs 组成。基因组的 G+C 含量为 72.4%。共预测了 7730 个基因，其中包括 2 个 rRNA 和 64 个 tRNA。DRQ-2 菌株的基因组分析显示存在广泛的次生代谢物基因簇。泛基因组分析管道（PGAP）表明，DRQ-2 菌株具有大量独特的基因。大多数 N. indica DRQ-2 基因编码假设蛋白，表明这些同源簇的功能仍有待确定。
