College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; Yunnan Institute of Fisheries Sciences, Kunming 650111, China.
College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China.
Gene. 2020 Jun 20;744:144632. doi: 10.1016/j.gene.2020.144632. Epub 2020 Mar 30.
IGF2BPs, a subclass of RNA-binding proteins, regulate cellular differentiation, proliferation and migration during multiple organs development, but their functions in liver development still remain unclear. Here, in this study, whole-mount in situ hybridization showed that igf2bp1 was constantly and stably expressed at early stages of embryo development in zebrafish. Both the morpholino-induced knockdown and CRISPR/Cas9-mediated knockout of igf2bp1 led to a reduced-size liver phenotype. Further analysis revealed that igf2bp1 is required for hepatic outgrowth, but not for hepatoblast specification and budding. Deficiency of igf2bp1 resulted in reduced cell proliferation, but had no effect on apoptosis. Therefore, we concluded that igf2bp1 is a critical factor to regulate hepatic outgrowth via cell proliferation during early liver development in zebrafish.
IGF2BPs 是 RNA 结合蛋白的一个子类，在多个器官的发育过程中调节细胞分化、增殖和迁移，但它们在肝脏发育中的功能尚不清楚。在这项研究中，整体原位杂交显示 igf2bp1 在斑马鱼胚胎发育的早期阶段持续且稳定地表达。IGF2BPs 的 Morpholino 诱导敲低和 CRISPR/Cas9 介导的敲除均导致肝脏体积减小的表型。进一步的分析表明，igf2bp1 对于肝芽的生长是必需的，但对于肝母细胞的特化和出芽不是必需的。igf2bp1 的缺失导致细胞增殖减少，但对细胞凋亡没有影响。因此，我们得出结论，igf2bp1 是通过调节细胞增殖来调控早期斑马鱼肝脏发育中肝芽生长的关键因素。
