van de Venter Maryna, Didloff Jenske, Reddy Shanika, Swanepoel Bresler, Govender Sharlene, Dambuza Ntokozo Shirley, Williams Saralene, Koekemoer Trevor Craig, Venables Luanne
Department of Biochemistry and Microbiology, Nelson Mandela University, PO Box 77000, Port Elizabeth 6031, South Africa.
Department of Pharmacy, Nelson Mandela University, PO Box 77000, Port Elizabeth 6031, South Africa.
Animals (Basel). 2020 Dec 30;11(1):54. doi: 10.3390/ani11010054.
Zebrafish have become a popular alternative to higher animals in biomedical and pharmaceutical research. The development of stable mutant lines to model target specific aspects of many diseases, including diabetes, is well reported. However, these mutant lines are much more costly and challenging to maintain than wild-type zebrafish and are simply not an option for many research facilities. As an alternative to address the disadvantages of advanced mutant lines, wild-type larvae may represent a suitable option. In this review, we evaluate organ development in zebrafish larvae and discuss established methods that use wild-type zebrafish larvae up to seven days post fertilization to test for potential drug candidates for diabetes and its commonly associated conditions of oxidative stress and inflammation. This provides an up to date overview of the relevance of wild-type zebrafish larvae as a vertebrate antidiabetic model and confidence as an alternative tool for preclinical studies. We highlight the advantages and disadvantages of established methods and suggest recommendations for future developments to promote the use of zebrafish, specifically larvae, rather than higher animals in the early phase of antidiabetic drug discovery.
斑马鱼已成为生物医学和药物研究中高等动物的一种流行替代模型。已有充分报道表明，开发稳定的突变系来模拟包括糖尿病在内的多种疾病的特定靶点方面。然而，与野生型斑马鱼相比，这些突变系的维护成本更高且更具挑战性，对于许多研究机构来说根本不是一个可行的选择。作为解决先进突变系缺点的一种替代方法，野生型幼虫可能是一个合适的选择。在这篇综述中，我们评估了斑马鱼幼虫的器官发育，并讨论了已建立的方法，这些方法使用受精后长达七天的野生型斑马鱼幼虫来测试糖尿病及其常见相关的氧化应激和炎症状况的潜在候选药物。这提供了野生型斑马鱼幼虫作为脊椎动物抗糖尿病模型的相关性的最新概述，并作为临床前研究的替代工具的可信度。我们强调了已建立方法的优缺点，并为未来的发展提出建议，以促进在抗糖尿病药物发现的早期阶段使用斑马鱼，特别是幼虫，而不是高等动物。
