Department of Physiology, Biosciences Institute, UNESP-São Paulo State University, CEP 18618-689, Botucatu, São Paulo, Brazil.
Department of Physiology, Biosciences Institute, UNESP-São Paulo State University, CEP 18618-689, Botucatu, São Paulo, Brazil; Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Alberta, Canada.
J Ethnopharmacol. 2020 Jun 28;256:112793. doi: 10.1016/j.jep.2020.112793. Epub 2020 Mar 30.
Terminalia catappa L. (Combretaceae), known as "amendoeira da praia" in Brazil, has been recognized as a medicinal plant in folk medicine for the treatment of gastrointestinal disorders and other inflammatory conditions. The present study aimed to investigate the preventive and healing effects of the infusion of leaves of T. catappa (ILTC) against gastric lesions caused by ischemia and reperfusion (I/R) injury and characterize its mechanism of action in the gastric mucosa of rats.
Different doses (30, 100, and 300 mg/kg) of ILTC were orally administered as acute and subacute treatments against I/R-induced gastric lesion in rats. After treatment, the stomach of rats was collected to measure the lesion area, redox parameters malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH) and inflammatory parameters myeloperoxidase activity (MPO), interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α). The activities of matrix metalloproteinases 2 and 9 (MMPs 2 and 9) were assessed by zymography method to clarify the mechanisms of the healing acceleration promoted by ILTC.
Pretreatment with ILTC (100 mg/kg) was effective in preventing the aggravation of lesions in the acute model by reducing MPO activity by 38% relative to control group, despite the lack of clarity of this action at the macroscopical level at the lesion area (p < 0.05). After three days of treatment with ILTC (30 and 100 mg/kg), this infusion significantly reduced the lesion area by 95% and 89%, respectively, compared the control (p < 0.05). The gastric healing effect of all doses of ILTC was followed by a reduction in MPO activity (decrease by 70-78%). Compared to the negative control, an improvement in gastric healing owing to treatment with ILTC was observed and this was followed by an increase in MMP-2 (20-47%) (p < 0.05).
Three days of treatment with ILTC could accelerate the healing process in I/R-induced lesions in rats. By decreasing MPO levels, ILTC enabled the action of MMP-2, which led to tissue recovery in the gastric mucosa.
Terminalia catappa L.（使君子科），在巴西被称为“amendoeira da praia”，已被民间医学认可为一种用于治疗胃肠道疾病和其他炎症性疾病的药用植物。本研究旨在探讨Terminalia catappa 叶浸剂（ILTC）对缺血再灌注（I/R）损伤引起的胃损伤的预防和治疗作用，并表征其在大鼠胃黏膜中的作用机制。
ILTC 的不同剂量（30、100 和 300mg/kg）作为急性和亚急性治疗，用于治疗大鼠 I/R 诱导的胃损伤。治疗后，收集大鼠的胃，测量损伤面积、氧化还原参数丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和还原型谷胱甘肽（GSH）以及炎症参数髓过氧化物酶活性（MPO）、白细胞介素 1β（IL-1β）和肿瘤坏死因子 α（TNF-α）。通过明胶酶谱法评估基质金属蛋白酶 2 和 9（MMPs 2 和 9）的活性，以阐明 ILTC 促进愈合加速的机制。
ILTC（100mg/kg）预处理可通过将 MPO 活性相对于对照组降低 38%来有效预防急性模型中损伤的加重，尽管在损伤面积的宏观水平上这种作用尚不清楚（p<0.05）。ILTC（30 和 100mg/kg）治疗 3 天后，与对照组相比，该浸剂分别显著降低了 95%和 89%的损伤面积（p<0.05）。所有剂量的 ILTC 的胃愈合作用均伴随着 MPO 活性的降低（降低 70-78%）。与阴性对照组相比，ILTC 治疗观察到胃愈合的改善，这伴随着 MMP-2 的增加（20-47%）（p<0.05）。
ILTC 治疗 3 天可加速大鼠 I/R 诱导损伤的愈合过程。通过降低 MPO 水平，ILTC 使 MMP-2 发挥作用，从而导致胃黏膜组织恢复。
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