Zanatta Ana C, Vieira Natália Carolina, Dantas-Medeiros Renato, Vilegas Wagner, Edrada-Ebel RuAngelie
Department of Biochemistry and Organic Chemistry, São Paulo State University (UNESP), Araraquara 14800-060, SP, Brazil.
Institute of Biosciences, São Paulo State University (UNESP), São Vicente 11380-972, SP, Brazil.
Metabolites. 2023 Feb 27;13(3):349. doi: 10.3390/metabo13030349.
L. (Combretaceae) is a medicinal plant that is part of the Brazilian biodiversity; this plant is popularly used for the treatment of a wide range of diseases. To better understand the chemical composition of in different seasons, we conducted a thorough study using LC-MS and NMR data analysis techniques. The study helped obtain a chemical profile of the plant ethanolic extracts in different seasons of the year (spring, summer, autumn, and winter). The dereplication of LC-HRMS data allowed the annotation of 90 compounds in the extracts of (hydrolyzable tannins, ellagic acid derivatives, and glycosylated flavonoids). Triterpenes and -glycosyl flavones were the compounds that significantly contributed to differences observed between plant samples harvested in autumn/winter and spring, respectively. The variations observed in the compound composition of the plant leaves may be related to processes induced by environmental stress and leaf development. Data fusion applied in the metabolomic profiling study allowed us to identify metabolites with greater confidence, and provided a better understanding regarding the production of specialized metabolites in leaves under different environmental conditions, which may be useful to establish appropriate quality criteria for the standardization of this medicinal plant.
L.（使君子科）是一种药用植物，属于巴西生物多样性的一部分；这种植物在民间广泛用于治疗多种疾病。为了更好地了解其在不同季节的化学成分，我们使用液相色谱 - 质谱联用（LC - MS）和核磁共振（NMR）数据分析技术进行了深入研究。该研究有助于获得该植物在一年中不同季节（春季、夏季、秋季和冬季）乙醇提取物的化学图谱。液相色谱 - 高分辨质谱（LC - HRMS）数据的去重复分析使得能够鉴定出该植物提取物中的90种化合物（可水解单宁、鞣花酸衍生物和糖基化黄酮类化合物）。三萜类化合物和β - 糖基黄酮分别是导致秋季/冬季和春季采集的该植物样本之间观察到差异的显著贡献化合物。在植物叶片化合物组成中观察到的变化可能与环境胁迫和叶片发育诱导的过程有关。代谢组学分析研究中应用的数据融合使我们能够更有信心地鉴定代谢物，并更好地了解在不同环境条件下该植物叶片中特殊代谢物的产生情况，这可能有助于为这种药用植物的标准化建立适当的质量标准。
