Centro de Química Estrutural, Departamento de Engenharia Química, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisboa, Portugal.
iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisboa, Portugal.
Colloids Surf B Biointerfaces. 2020 Jul;191:110990. doi: 10.1016/j.colsurfb.2020.110990. Epub 2020 Mar 27.
Zinc coated with nanostructured ZnO flowers has received increasing attention as a versatile biomaterial for medical applications. Whatsoever, the potential of these materials to meet specific medical requirements must be explored. Despite in its infancy, surface functionalization is the key strategy to achieve this goal. The functionalization, successfully achieved with cooper (Cu), iron (Fe) or manganese (Mn) oxides (Ox), was highly dependent on the presence of the flowered structures, with the deep physicochemical characterization of these new surfaces revealing specific metal oxide distributions. The functionalization with these metal oxides resulted in distinct biological and in vitro behaviours. The biological response, assessed by fibroblast viability, hemocompatibility, and chick chorioallantoic membrane (CAM), further supported by the in vitro degradation studies, evaluated by immersion and electrochemical techniques, revealed that the deleterious role of CuOx functionalization brought potential for anti-cancer applications; with an antagonist behaviour, the functionalization with MnOx, and in a less extent with FeOx, can be used to favour wound healing in traumatic processes. Despite the possible correlation between biocompatibility and hydroxyapatite precipitation, no correlation could be drawn with the corrosion activity of these surfaces. Overall, the minor addition of relevant physiological as Cu, Fe or Mn oxides resulted in antagonist in vitro responses that can be used as expedite strategies to modulate the behaviour of Zn-based materials, contributing in this way for the design of anti-cancer or wound healing therapies.
具有纳米结构 ZnO 花的镀锌层作为一种用于医疗应用的多功能生物材料受到了越来越多的关注。然而，必须探索这些材料是否具有满足特定医疗需求的潜力。尽管处于起步阶段，但表面功能化是实现这一目标的关键策略。成功地用铜 (Cu)、铁 (Fe) 或锰 (Mn) 氧化物 (Ox) 进行的功能化高度依赖于花状结构的存在，对这些新表面的深入物理化学特性研究揭示了特定金属氧化物的分布。用这些金属氧化物进行功能化导致了明显不同的生物学和体外行为。通过成纤维细胞活力、血液相容性和鸡胚绒毛尿囊膜 (CAM) 评估的生物学反应，进一步通过浸泡和电化学技术进行的体外降解研究得到支持，表明 CuOx 功能化的有害作用为抗癌应用带来了潜力; 具有拮抗作用，MnOx 的功能化，以及在较小程度上 FeOx 的功能化，可用于促进创伤过程中的伤口愈合。尽管生物相容性和羟基磷灰石沉淀之间可能存在相关性，但这些表面的腐蚀活性与生物相容性之间没有相关性。总的来说，少量添加相关的生理如 Cu、Fe 或 Mn 氧化物会导致体外拮抗反应，可作为调节 Zn 基材料行为的加速策略，从而为设计抗癌或伤口愈合疗法做出贡献。
