Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, China.
J Plant Physiol. 2020 May;248:153158. doi: 10.1016/j.jplph.2020.153158. Epub 2020 Mar 24.
Lysine acetylation is one of the most important post-translational modifications and is involved in multiple cellular processes in plants. There is evidence that acetylation may play an important role in light-induced de-etiolation, a key developmental switch from skotomorphogenesis to photomorphogenesis. During this transition, establishment of photosynthesis is of great significance. However, studies on acetylome dynamics during de-etiolation are limited. Here, we performed the first global lysine acetylome analysis for Zea mays seedlings undergoing de-etiolation, using nano liquid chromatography coupled to tandem mass spectrometry, and identified 814 lysine-acetylated sites on 462 proteins. Bioinformatics analysis of this acetylome showed that most of the lysine-acetylated proteins are predicted to be located in the cytoplasm, nucleus, chloroplast, and mitochondria. In addition, we detected ten lysine acetylation motifs and found that the accumulation of 482 lysine-acetylated peptides corresponding to 289 proteins changed significantly during de-etiolation. These proteins include transcription factors, histones, and proteins involved in chlorophyll synthesis, photosynthesis light reaction, carbon assimilation, glycolysis, the TCA cycle, amino acid metabolism, lipid metabolism, and nucleotide metabolism. Our study provides an in-depth dataset that extends our knowledge of in vivo acetylome dynamics during de-etiolation in monocots. This dataset promotes our understanding of the functional consequences of lysine acetylation in diverse cellular metabolic regulatory processes, and will be a useful toolkit for further investigations of the lysine acetylome and de-etiolation in plants.
赖氨酸乙酰化是最重要的翻译后修饰之一，参与植物的多种细胞过程。有证据表明，乙酰化可能在光诱导去黄化中发挥重要作用，这是一个从暗形态建成到光形态建成的关键发育开关。在这个转变过程中，光合作用的建立具有重要意义。然而，关于去黄化过程中乙酰化组动态变化的研究还很有限。在这里，我们使用纳升液相色谱串联质谱法，对正在去黄化的玉米幼苗进行了首次全局赖氨酸乙酰化组分析，鉴定了 462 个蛋白质上的 814 个赖氨酸乙酰化位点。对该乙酰化组的生物信息学分析表明，大多数赖氨酸乙酰化蛋白被预测位于细胞质、细胞核、叶绿体和线粒体中。此外，我们检测到十个赖氨酸乙酰化基序，并发现 289 个蛋白质中 482 个赖氨酸乙酰化肽的积累在去黄化过程中发生了显著变化。这些蛋白质包括转录因子、组蛋白和参与叶绿素合成、光合作用光反应、碳同化、糖酵解、三羧酸循环、氨基酸代谢、脂质代谢和核苷酸代谢的蛋白质。我们的研究提供了一个深入的数据集，扩展了我们对单子叶植物去黄化过程中体内乙酰化组动态变化的认识。该数据集促进了我们对赖氨酸乙酰化在不同细胞代谢调节过程中的功能后果的理解，并将成为进一步研究植物赖氨酸乙酰化组和去黄化的有用工具包。
