荧光团诱导等离子体电流:基于生成的单线态氧检测。

Fluorophore-Induced Plasmonic Current: Generation-Based Detection of Singlet Oxygen.

机构信息

Institute of Fluorescence and Department of Chemistry and Biochemistry, University of Maryland, Baltimore County, 701 East Pratt Street, Baltimore, Maryland 21202, United States.

出版信息

ACS Sens. 2020 Apr 24;5(4):1223-1229. doi: 10.1021/acssensors.0c00377. Epub 2020 Apr 14.

Abstract

In this work, we report the surface-based electrical detection of singlet oxygen using the emerging fluorophore-induced plasmonic current (PC) technique. By this method, we utilize the fluorescent "turn on" response of the well-known singlet oxygen sensor green (SOSG) singlet oxygen (O) fluorescent probe for the generation of fluorophore-induced PC in a silver nanoparticle film. To demonstrate the potential utility of this new technique, a photosensitizing molecule is used to generate O in a solution containing the SOSG probe. The resulting change in SOSG fluorescence quantum yield and extinction coefficient permits stronger energy transfer from the SOSG probe to a proximal silver nanoparticle island film located in the near-electric field of the probe. This yields an increase in the induced electric current flow, allowing for the detection of the O analyte. To the author's knowledge, this represents the first detection of the reactive oxygen species O utilizing fluorophore-induced PC methodology and even broader electrical detection of O This is significant as it opens the possibility for O detection methods which do not require a traditional "photodetector" and associated optics, simplifying the instrumentation over existing fluorescence detection methods and potentially even lowering the cost.

摘要

在这项工作中,我们报告了使用新兴的荧光团诱导等离子体电流(PC)技术对单线态氧进行基于表面的电检测。通过这种方法,我们利用众所周知的单线态氧传感器绿(SOSG)单线态氧(O)荧光探针的荧光“开启”响应,在银纳米粒子薄膜中产生荧光团诱导的 PC。为了证明这项新技术的潜在应用价值,我们使用光敏分子在含有 SOSG 探针的溶液中产生 O。SOSG 荧光量子产率和消光系数的变化允许从 SOSG 探针更强烈地向位于探针近电场中的邻近银纳米岛薄膜进行能量转移。这导致感应电流的增加,从而可以检测到 O 分析物。据作者所知,这代表了首次利用荧光团诱导 PC 方法检测活性氧物种 O,甚至更广泛地检测 O。这很重要,因为它为 O 检测方法开辟了可能性,这些方法不需要传统的“光电探测器”和相关光学器件,简化了现有荧光检测方法的仪器设备,甚至可能降低成本。

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