An Jong Min, Kim Sung Hyun, Kim Dokyoung
Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul 02447, Republic of Korea.
Org Biomol Chem. 2020 Apr 3. doi: 10.1039/d0ob00515k.
Two-photon microscopy (TPM) techniques have been highlighted over the past two decades throughout various fields, including physics, chemistry, biology, and medicine. In particular, the two-photon near-infrared excitation of fluorophores or molecular probes emitting fluorescence have ushered in a new biomedical era, specifically in the deep-tissue imaging of biologically relevant species. Non-linear two-photon optics enables the development of 3D fluorescence images via focal point excitation of biological samples with low photo-damage and photo-bleaching. Many studies have disclosed the relationship between the chemical structure of fluorophores and their two-photon absorbing properties. In this review, we have summarized the recent advances in two-photon absorbing probes based on a functionalized electron donor (D)-acceptor (A) type dipolar naphthalene platform (FDNP) that was previously reported between 2015 and 2019. Our systematic outline of the synthesis, photophysical properties, and examples of two-photon imaging applications will provide useful context for the future development of new naphthalene backbone-based two-photon probes.
在过去二十年中，双光子显微镜（TPM）技术在包括物理、化学、生物学和医学在内的各个领域都备受关注。特别是，荧光团或发射荧光的分子探针的双光子近红外激发开启了一个新的生物医学时代，尤其是在对生物相关物种的深层组织成像方面。非线性双光子光学通过对生物样品进行焦点激发，能够在低光损伤和光漂白的情况下生成三维荧光图像。许多研究揭示了荧光团的化学结构与其双光子吸收特性之间的关系。在本综述中，我们总结了基于功能化电子供体（D）-受体（A）型偶极萘平台（FDNP）的双光子吸收探针的最新进展，该平台在2015年至2019年期间已有相关报道。我们对合成、光物理性质以及双光子成像应用实例的系统概述，将为未来基于萘骨架的新型双光子探针的开发提供有用的背景信息。
