Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha 410082, People's Republic of China.
Methods Appl Fluoresc. 2017 Mar 22;5(1):012003. doi: 10.1088/2050-6120/aa61b0.
During the past two decades, two-photon microscopy (TPM), which utilizes two near-infrared photons as the excitation source, has emerged as a novel, attractive imaging tool for biological research. Compared with one-photon microscopy, TPM offers several advantages, such as lowering background fluorescence in living cells and tissues, reducing photodamage to biosamples, and a photobleaching phenomenon, offering better 3D spatial localization, and increasing penetration depth. Small-molecule-based two-photon fluorescent probes have been well developed for the detection and imaging of various analytes in biological systems. In this review, we will give a general introduction of molecular engineering of two-photon fluorescent probes based on different fluorescence response mechanisms for bioimaging applications during the past decade. Inspired by the desired advantages of small-molecule two-photon fluorescent probes in biological imaging applications, we expect that more attention will be devoted to the development of new two-photon fluorophores and applications of TPM in areas of bioanalysis and disease diagnosis.
在过去的二十年中，双光子显微镜（TPM）作为一种新型的、有吸引力的生物研究成像工具，已经崭露头角。与单光子显微镜相比，TPM 具有几个优势，如降低活细胞和组织中的背景荧光、减少对生物样本的光损伤和光漂白现象，提供更好的 3D 空间定位，并增加穿透深度。基于不同的荧光响应机制，小分子双光子荧光探针已被很好地开发出来，用于检测和成像生物体系中的各种分析物。在这篇综述中，我们将对过去十年中基于不同荧光响应机制的用于生物成像应用的双光子荧光探针的分子工程进行综述。受小分子双光子荧光探针在生物成像应用中的理想优势的启发，我们期望更多的注意力将致力于新型双光子荧光团的开发和 TPM 在生物分析和疾病诊断领域的应用。
