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通过溴酚蓝与 CsgA 之间的超分子识别对细菌卷曲菌和生物膜进行活细胞荧光染色。

Live cell fluorescent stain of bacterial curli and biofilm through supramolecular recognition between bromophenol blue and CsgA.

机构信息

State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, College of Chemistry and International Joint Research Laboratory of Nano-Micro Architecture Chemistry, Jilin University, Changchun 130012, China.

出版信息

Chem Commun (Camb). 2020 May 7;56(37):5014-5017. doi: 10.1039/d0cc01643h. Epub 2020 Apr 3.

Abstract

Identification of curli-specific dyes for biofilm communities of microorganisms is an important task. We describe here a curli fluorescent light-up probe called bromophenol blue, which binds to curli via recognizing CsgA. This platform may provide a new perspective for the research on biofilm, amyloid disease and living materials.

摘要

鉴定微生物生物膜群落中卷曲菌特异性染料是一项重要任务。我们在这里描述了一种卷曲菌荧光点亮探针,称为溴酚蓝,它通过识别 CsgA 与卷曲菌结合。该平台可能为生物膜、淀粉样变性疾病和活体材料的研究提供新的视角。

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