Faculty of Veterinary Medicine, Autonomous University of Tamaulipas, Victoria, Mexico.
Institute of Veterinary Science Research, Autonomous University of Baja California, Mexicali, Mexico.
Protein Pept Lett. 2021;28(1):101-107. doi: 10.2174/0929866527666200403111743.
Bovine Lactoferrin (bLf) has been reported as antimicrobial, antiviral, immunomodulatory and anticancer protein. Escherichia coli and Listeria spp. are food-borne bacteria that can produce illness in human being and mammals, the emergent antimicrobial drug resistance has been reported in these pathogens.
The aim for this study was to evaluate the bLf effect on in vitro biofilm production and the synergic effect of antibiotics on E. coli and Listeria isolates.
E. coli and Listeria specimens were isolated from bovine carcasses and slaughterhouses surfaces, respectively. Biofilm formation was analyzed with or without bLf, incubated for 48 h and spectrophotometry, cell viability was analyzed by colony-forming unit (CFU) and the synergistic effect of bLf with ampicillin, oxytetracycline, and streptomycin was evaluated through the fractional concentration index (FCI).
Our results show that a low bLf concentration (0.8 μM) can diminish the in vitro biofilm production in Listeria isolates; also improves the in vitro oxytetracycline and streptomycin activity against E. coli, and ampicillin activity against Listeria isolates.
bLf can affect the biofilm production in Listeria isolates from slaughterhouses surfaces and shown synergic effect with ampicillin. Also has a synergic effect with oxytetracycline and streptomycin against E. coli isolates from bovine carcasses.
牛乳铁蛋白（bLf）已被报道具有抗菌、抗病毒、免疫调节和抗癌作用。大肠杆菌和李斯特菌是食源性细菌，会导致人类和哺乳动物患病，这些病原体已报告出现抗药性。
本研究旨在评估 bLf 对大肠杆菌和李斯特菌分离株体外生物膜形成的影响以及抗生素的协同作用。
从牛尸体和屠宰场表面分离大肠杆菌和李斯特菌标本。通过分光光度法分析有无 bLf 孵育 48 小时后的生物膜形成情况，通过集落形成单位（CFU）分析细胞活力，并通过部分浓度指数（FCI）评估 bLf 与氨苄西林、土霉素和链霉素的协同作用。
我们的结果表明，低浓度 bLf（0.8 μM）可减少李斯特菌分离株的体外生物膜形成；还可提高体外土霉素和链霉素对大肠杆菌的活性，以及氨苄西林对李斯特菌分离株的活性。
bLf 可影响屠宰场表面李斯特菌分离株的生物膜形成，并与氨苄西林表现出协同作用。对牛尸体分离的大肠杆菌也表现出与土霉素和链霉素的协同作用。
