酪蛋白激酶I和II与猪脑微管结合。
Casein kinases I and II bound to pig brain microtubules.
作者信息
Risnik V V, Adám G, Gusev N B, Friedrich P
机构信息
Department of Biochemistry, School of Biology, Moscow State University, U.S.S.R.
出版信息
Cell Mol Neurobiol. 1988 Sep;8(3):315-24. doi: 10.1007/BF00711173.
Abstract
- Microtubules prepared from pig brain by two cycles of assembly-disassembly comprise cyclic nucleotide-independent protein kinase activity with phosvitin and troponin T as substrates. 2. Phosphocellulose chromatography resolved two phosvitin kinase activity peaks, one of which coincided with the troponin T kinase peak. 3. The activity peak corresponding to troponin T kinase was inhibited by heparin (I50 = 0.06 micrograms/ml), whereas the other phosvitin kinase peak was unaffected. 4. Both kinase fractions phosphorylated tubulin and microtubule-associated protein (MAP-2). 5. It is concluded that pig brain microtubules contain bound casein kinases I and II. The association may target the action of these kinases toward microtubular proteins in vivo.
摘要
- 通过两轮组装-拆卸从猪脑中制备的微管包含以磷蛋白和肌钙蛋白T为底物的不依赖环核苷酸的蛋白激酶活性。2. 磷酸纤维素色谱法分离出两个磷蛋白激酶活性峰，其中一个与肌钙蛋白T激酶峰重合。3. 对应于肌钙蛋白T激酶的活性峰被肝素抑制（I50 = 0.06微克/毫升），而另一个磷蛋白激酶峰不受影响。4. 两个激酶组分均使微管蛋白和微管相关蛋白（MAP-2）磷酸化。5. 得出的结论是，猪脑微管含有结合的酪蛋白激酶I和II。这种结合可能在体内使这些激酶的作用靶向微管蛋白。
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