三碘甲状腺原氨酸对原代培养大鼠下颌下腺细胞静息膜电位的影响。

Effects of triiodothyronine on resting membrane potential of primary cultured rat submandibular gland cells.

作者信息

Bryant H J, Eng S P, Klein L E, Lo C S

机构信息

Department of Physiology, Uniformed Services University of the Health Sciences, Hebert School of Medicine, Bethesda, MD 20814-4799.

出版信息

Cell Biol Int Rep. 1988 Dec;12(12):1027-36. doi: 10.1016/0309-1651(88)90027-6.

DOI:10.1016/0309-1651(88)90027-6

PMID:3224376

Abstract

The effect of triiodothyronine (T3) on the resting membrane potential was measured in primary cultured rat submandibular gland cells. The resting membrane potential was 29.5 +/- 0.71 mV. The hormone T3, at concentrations of 10(-9) M or greater, hyperpolarized the cells 5.8 mV (p less than 0.05). Hyperpolarization was complete within 24 hours. Ouabain (1 mM) depolarized the cells 5.9 mV. Cells exposed to T3 and ouabain had the same membrane potential as cells treated with ouabain alone. These data suggest that the hyperpolarization observed can be, in part, attributed to triiodothyronine-induced synthesis of (Na-K)-adenosine triphosphatase.

摘要

在原代培养的大鼠下颌下腺细胞中测量了三碘甲状腺原氨酸（T3）对静息膜电位的影响。静息膜电位为29.5±0.71 mV。浓度为10^(-9) M或更高的激素T3使细胞超极化5.8 mV（p<0.05）。超极化在24小时内完成。哇巴因（1 mM）使细胞去极化5.9 mV。暴露于T3和哇巴因的细胞与仅用哇巴因处理的细胞具有相同的膜电位。这些数据表明，观察到的超极化部分可归因于三碘甲状腺原氨酸诱导的（钠-钾）-三磷酸腺苷酶的合成。