CERVO Brain Research Center, Quebec City, QC G1J 2G3, Canada.
CERVO Brain Research Center, Quebec City, QC G1J 2G3, Canada; Department of Psychiatry and Neuroscience, Université Laval, Quebec City, QC G1K 7P4, Canada.
Stem Cell Reports. 2020 Apr 14;14(4):692-702. doi: 10.1016/j.stemcr.2020.03.008. Epub 2020 Apr 2.
The neural stem cells (NSCs) residing in the olfactory epithelium (OE) regenerate damaged olfactory sensory neurons throughout adulthood. The accessibility and availability of these NSCs in living individuals, including humans, makes them a promising candidate for harvesting their potential for cell replacement therapies. However, this requires an in-depth understanding of their developmental potential after grafting. Here, we investigated the developmental potential and plasticity of mouse OE-derived NSCs after grafting into the adult subventricular zone (SVZ) neurogenic niche. Our results showed that OE-derived NSCs integrate and proliferate just like endogenous SVZ stem cells, migrate with similar dynamics as endogenous neuroblasts toward the olfactory bulb, and mature and acquire similar electrophysiological properties as endogenous adult-born bulbar interneurons. These results reveal the developmental potential and plasticity of OE-derived NSCs in vivo and show that they can respond to heterotopic neurogenic cues to adapt their phenotype and become functional neurons in ectopic brain regions.
神经干细胞（NSCs）存在于嗅上皮（OE）中，可在成年期再生受损的嗅觉感觉神经元。这些 NSCs 在活体中，包括人类中的可及性和可用性，使它们成为用于收获其细胞替代治疗潜力的有希望的候选物。然而，这需要深入了解它们在移植后的发育潜力。在这里，我们研究了在成年侧脑室下区（SVZ）神经发生龛中移植后，小鼠 OE 衍生 NSCs 的发育潜力和可塑性。我们的结果表明，OE 衍生的 NSCs 与内源性 SVZ 干细胞一样整合和增殖，与内源性神经母细胞一样以相似的动力学迁移到嗅球，并成熟并获得与内源性成年新生嗅球中间神经元相似的电生理特性。这些结果揭示了 OE 衍生 NSCs 在体内的发育潜力和可塑性，并表明它们可以响应异位神经发生信号来适应其表型，并在异位脑区成为功能性神经元。
