School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
Food Chem. 2020 Aug 15;321:126697. doi: 10.1016/j.foodchem.2020.126697. Epub 2020 Mar 26.
Based on colloidal gold and broad-spectrum monoclonal antibody that binds to zeranol and its five analogues with high sensitivity, a lateral flow immunochromatographic assay (LFIA) in a competitive format was developed to specifically determine residues of zeranol, an illegal growth promoter in livestock. In this study, the assay had high sensitivity and was broad-spectrum only for zeranol and its five analogues, and the results were obtained within 10 min without needing sophisticated procedures. The cutoff values for zeranol and its five analogues were 10 ng/mL, and the IC values for zeranol, β-zearalanol, zearalanone, α-zearalenol, β-zearalenol and zearalenone were 1.250, 1.800, 1.775, 1.225, 1.709 and 1.319 ng/mL, respectively. The recovery rates were ranged from 85.6 to 93.9%, with the coefficient of variations less than 12.4%. The results demonstrated that the LFIA could be used for rapid, simultaneous, semi-quantitative and quantitative detection of residues of zeranol and its five analogous in milk.
基于胶体金和广谱单克隆抗体，该抗体与玉米赤霉醇及其五种类似物具有高灵敏度结合，开发了一种侧向流动免疫层析测定法（LFIA），以特异性测定玉米赤霉醇的残留量，玉米赤霉醇是一种非法的牲畜生长促进剂。在这项研究中，该测定法具有高灵敏度，仅对玉米赤霉醇及其五种类似物具有广谱性，并且结果在 10 分钟内获得，无需复杂的程序。玉米赤霉醇及其五种类似物的截止值为 10 ng/mL，玉米赤霉醇、β-玉米赤霉醇、玉米赤霉酮、α-玉米赤霉醇、β-玉米赤霉醇和玉米赤霉烯酮的 IC 值分别为 1.250、1.800、1.775、1.225、1.709 和 1.319 ng/mL。回收率范围为 85.6%至 93.9%，变异系数小于 12.4%。结果表明，该 LFIA 可用于快速、同时、半定量和定量检测牛奶中玉米赤霉醇及其五种类似物的残留量。
