Zhuang Xiaoxin, Wang Zhiyan, Peng Chenghui, Su Can, Gao Congting, Wang Yongjiang, Huang Shengxiong, Liu Chongxi
Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin 150030, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Microorganisms. 2020 Mar 31;8(4):495. doi: 10.3390/microorganisms8040495.
A novel actinomycete producing heliquinomycin and 9'-methoxy-heliquinomycin, designated strain jys28, was isolated from rhizosphere soil of and characterized using a polyphasic approach. The strain had morphological characteristics and chemotaxonomic properties identical to those of members of the genus . It formed spiral chains of spores with spiny surfaces. The menaquinones detected were MK-9(H), MK-9(H) and MK-9(H). The major fatty acids were iso-C, C, Cω7с and anteiso-C. The phospholipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and phosphatidylinositol mannoside. The DNA G + C content of the draft genome sequence, consisting of 8.5 Mbp, was 70.6%. Analysis of the 16S rRNA gene sequence showed that strain jys28 belongs to the genus with the highest sequence similarities to NBRC 13058 (99.2%) and DSM 40002 (99.2%) and phylogenetically clustered with them. Multilocus sequence analysis based on five other house-keeping genes (D, B, B, A and B and the low level of DNA-DNA relatedness and phenotypic differences allowed the novel isolate to be differentiated from its most closely related strains. Therefore, the strain is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. Furthermore, the putative biosynthetic gene cluster of heliquinomycins was identified and the biosynthetic pathway was discussed. The type strain is jys28 (=CCTCC AA 2018051 =DSM 109823).
从[具体植物名称]根际土壤中分离出一株产日光霉素和9'-甲氧基-日光霉素的新型放线菌，命名为菌株jys28，并采用多相分类法对其进行了表征。该菌株具有与[属名]属成员相同的形态特征和化学分类学特性。它形成了表面有刺的螺旋状孢子链。检测到的甲基萘醌为MK-9(H)、MK-9(H)和MK-9(H)。主要脂肪酸为异-C、C、Cω7с和anteiso-C。磷脂为双磷脂酰甘油、磷脂酰甲基乙醇胺、磷脂酰乙醇胺和磷脂酰肌醇甘露糖苷。由8.5 Mbp组成的基因组草图序列的DNA G + C含量为70.6%。16S rRNA基因序列分析表明，菌株jys28属于[属名]属，与[菌株名称1]NBRC 13058(99.2%)和[菌株名称2]DSM 40002(99.2%)的序列相似性最高，并在系统发育上与其聚类。基于其他五个管家基因(D、B、B、A和B)进行的多位点序列分析以及低水平的DNA-DNA相关性和表型差异，使得该新分离菌株能够与其最密切相关的菌株区分开来。因此，得出该菌株代表[属名]属一个新物种的结论，并提出其名称为[新物种名称] sp. nov.。此外，还鉴定了日光霉素的假定生物合成基因簇并讨论了其生物合成途径。模式菌株为jys28(=CCTCC AA 2018051 =DSM 109823)。
需注意，原文中部分植物名称等信息缺失，我用[具体植物名称]、[属名]、[菌株名称1]、[菌株名称2]、[新物种名称]进行了标注。
