sp. nov., a novel actinomycete isolated from rhizosphere soil of rice ( L.).

A novel actinobacterium, designated strain NEAU-C40, was isolated from the rhizosphere soil of rice ( L.) collected from Northeast Agricultural University in Harbin, Heilongjiang province, north-east PR China, and was characterized using a polyphasic approach. On the basis of results of 16S rRNA gene sequence analysis, strain NEAU-C40 belongs to the genus , and shares highest sequence similarities with CGMCC 4.7237 (97.9%) and DSM 42080 (97.9%). Morphological and chemotaxonomic characteristics of the strain also supported its assignment to the genus . Cell walls contained ll-diaminopimelic acid and the whole-cell hydrolysates were glucose, rhamnose and ribose. The major menaquinones were identified as MK-9(H) and MK-9(H). The major fatty acids were iso-C, anteiso-C, C and anteiso-C. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and an unidentified lipid. The genomic DNA G+C content of strain NEAU-C40 was 71.8 mol%. Moreover, multilocus sequence analysis based on five other housekeeping genes ( , , , and ) and the low level of DNA-DNA relatedness allowed the isolate to be differentiated from its most closely related strains. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-C40 can be characterized to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-C40 (=DSM 107943=CCTCC AA 2018038).