College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Int J Mol Sci. 2020 Mar 31;21(7):2433. doi: 10.3390/ijms21072433.
JAZ (Jasmonate ZIM-domain) proteins play pervasive roles in plant development and defense reaction. However, limited information is known about the JAZ family in . In this study, 12 non-redundant genes were identified from the tea plant genome database. Phylogenetic analysis showed that the 12 JAZ proteins belong to three groups. The -elements in promoters of CsJAZ genes and CsJAZ proteins interaction networks were also analyzed. Quantitative RT-PCR analysis showed that 7 genes were preferentially expressed in roots. Furthermore, the expressions were differentially induced by cold, heat, polyethylene glycol (PEG), methyl jasmonate (MeJA), and gibberellin (GA) stimuli. The Pearson correlations analysis based on expression levels showed that the gene pairs were differentially expressed under different stresses, indicating that might exhibit synergistic effects in response to various stresses. Subcellular localization assay demonstrated that CsJAZ3, CsJAZ10, and CsJAZ11 fused proteins were localized in the cell nucleus. Additionally, the overexpression of , and in enhanced the growth of recombinant cells under abiotic stresses. In summary, this study will facilitate the understanding of the family in and provide new insights into the molecular mechanism of tea plant response to abiotic stresses and hormonal stimuli.
茉莉酸 ZIM 结构域蛋白（JAZ）在植物发育和防御反应中发挥着普遍作用。然而，关于茶树中的 JAZ 家族，人们知之甚少。在本研究中，从茶树基因组数据库中鉴定出 12 个非冗余基因。系统发育分析表明，这 12 个 JAZ 蛋白分为 3 组。还分析了 CsJAZ 基因启动子中的 -元件和 CsJAZ 蛋白互作网络。定量 RT-PCR 分析表明，7 个基因在根中优先表达。此外，这些基因的表达受到冷、热、聚乙二醇（PEG）、茉莉酸甲酯（MeJA）和赤霉素（GA）刺激的差异诱导。基于表达水平的 Pearson 相关性分析表明，在不同胁迫下这些基因对的表达存在差异，表明它们可能在应对各种胁迫时表现出协同作用。亚细胞定位试验表明，CsJAZ3、CsJAZ10 和 CsJAZ11 融合蛋白定位于细胞核。此外，在非生物胁迫下，过表达 、 和 增强了重组细胞的生长。综上所述，本研究将有助于理解茶树中的 家族，并为茶树应对非生物胁迫和激素刺激的分子机制提供新的见解。
