School of Pharmacy, Henan University of Chinese Medicine, 156 Esat Jin-shui Rd, Zhengzhou, 450046, PR China.
Henan Key Laboratory of Chinese Medicine Resources and Chemistry, Zhengzhou, 450046, PR China.
BMC Genomics. 2024 Oct 3;25(1):925. doi: 10.1186/s12864-024-10856-4.
Plant-specific TIFY proteins play crucial roles in regulating plant growth, development, and various stress responses. However, there is no information available about this family in Artemisia argyi, a well-known traditional medicinal plant with great economic value.
A total of 34 AaTIFY genes were identified, including 4 TIFY, 22 JAZ, 5 PPD, and 3 ZML genes. Structural, motif scanning, and phylogenetic relationships analysis of these genes revealed that members within the same group or subgroup exhibit similar exon-intron structures and conserved motif compositions. The TIFY genes were unevenly distributed across the 15 chromosomes. Tandem duplication events and segmental duplication events have been identified in the TIFY family in A. argyi. These events have played a crucial role in the gene multiplication and compression of different subfamilies within the TIFY family. Promoter analysis revealed that most AaTIFY genes contain multiple cis-elements associated with stress response, phytohormone signal transduction, and plant growth and development. Expression analysis of roots and leaves using RNA-seq data revealed that certain AaTIFY genes showed tissue-specific expression patterns, and some AaTIFY genes, such as AaTIFY19/29, were found to be involved in regulating salt and saline-alkali stresses. In addition, RT-qPCR analysis showed that TIFY genes, especially AaTIFY19/23/27/29, respond to a variety of hormonal treatments, such as MeJA, ABA, SA, and IAA. This suggested that TIFY genes in A. argyi regulate plant growth and respond to different stresses by following different hormone signaling pathways.
Taken together, our study conducted a comprehensive identification and analysis of the TIFY gene family in A. argyi. These findings suggested that TIFY might play an important role in plant development and stress responses, which laid a valuable foundation for further understanding the function of TIFY genes in multiple stress responses and phytohormone crosstalk in A. argyi.
植物特异性 TIFY 蛋白在调控植物生长、发育和各种胁迫响应中起着至关重要的作用。然而，在具有巨大经济价值的传统药用植物艾草中，关于这个家族的信息尚未可知。
共鉴定出 34 个 AaTIFY 基因，包括 4 个 TIFY、22 个 JAZ、5 个 PPD 和 3 个 ZML 基因。对这些基因的结构、基序扫描和系统发育关系分析表明，同一组或亚组内的成员具有相似的外显子-内含子结构和保守的基序组成。TIFY 基因在 15 条染色体上不均匀分布。在艾草的 TIFY 家族中发现了串联重复事件和片段重复事件。这些事件在不同亚家族的基因倍增和压缩中发挥了关键作用。启动子分析表明，大多数 AaTIFY 基因含有多个与应激响应、植物激素信号转导以及植物生长和发育相关的顺式元件。使用 RNA-seq 数据对根和叶进行的表达分析表明，某些 AaTIFY 基因表现出组织特异性表达模式，并且发现一些 AaTIFY 基因，如 AaTIFY19/29，参与调控盐和盐碱胁迫。此外，RT-qPCR 分析表明，TIFY 基因，特别是 AaTIFY19/23/27/29，对多种激素处理（如 MeJA、ABA、SA 和 IAA）有响应。这表明艾草中的 TIFY 基因通过不同的激素信号通路调节植物生长并对不同胁迫做出响应。
综上所述，本研究对艾草中的 TIFY 基因家族进行了全面的鉴定和分析。这些发现表明 TIFY 可能在植物发育和胁迫响应中发挥重要作用，为进一步理解 TIFY 基因在艾草中多种胁迫响应和植物激素互作中的功能奠定了基础。
