The College of Forestry, Beijing Forestry University, Beijing 100083, China.
State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, and School of Advanced Agricultural Sciences, Peking University, Beijing 100871, China.
Genes (Basel). 2020 Apr 1;11(4):380. doi: 10.3390/genes11040380.
Pigeonpea is an important economic crop in the world and is mainly distributed in tropical and subtropical regions. In order to further expand the scope of planting, one of the problems that must be solved is the impact of soil acidity on plants in these areas. Based on our previous work, we constructed a time series RNA sequencing (RNA-seq) analysis under aluminum (Al) stress in pigeonpea. Through a comparison analysis, 11,425 genes were found to be differentially expressed among all the time points. After clustering these genes by their expression patterns, 12 clusters were generated. Many important functional pathways were identified by gene ontology (GO) analysis, such as biological regulation, localization, response to stimulus, metabolic process, detoxification, and so on. Further analysis showed that metabolic pathways played an important role in the response of Al stress. Thirteen out of the 23 selected genes related to flavonoids and phenols were downregulated in response to Al stress. In addition, we verified these key genes of flavonoid- and phenol-related metabolism pathways by qRT-PCR. Collectively, our findings not only revealed the regulation mechanism of pigeonpea under Al stress but also provided methodological support for further exploration of plant stress regulation mechanisms.
木豆是一种重要的经济作物，在世界范围内广泛种植，主要分布在热带和亚热带地区。为了进一步扩大种植范围，必须解决的问题之一是这些地区土壤酸度对植物的影响。基于我们之前的工作，我们构建了木豆在铝胁迫下的时间序列 RNA 测序（RNA-seq）分析。通过对比分析，在所有时间点共发现 11425 个差异表达基因。通过对这些基因的表达模式进行聚类，共生成 12 个簇。基因本体（GO）分析鉴定了许多重要的功能途径，如生物调节、定位、对刺激的反应、代谢过程、解毒等。进一步的分析表明，代谢途径在铝胁迫响应中起着重要作用。与类黄酮和酚类相关的 23 个选定基因中有 13 个在铝胁迫下下调。此外，我们还通过 qRT-PCR 验证了这些与类黄酮和酚类代谢途径相关的关键基因。总之，我们的研究结果不仅揭示了木豆在铝胁迫下的调控机制，还为进一步探索植物胁迫调控机制提供了方法学支持。
