抑制 nbe-miR1919c-5p 的表达可增强烟草曲叶病毒及其卫星β的共感染。
Suppression of nbe-miR1919c-5p Expression in Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection.
机构信息
Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, China.
出版信息
Viruses. 2020 Apr 1;12(4):392. doi: 10.3390/v12040392.
MicroRNAs (miRNAs) are non-coding but functional RNA molecules of 21-25 nucleotides in length. MiRNAs play significant regulatory roles in diverse plant biological processes. In order to decipher the relationship between nbe-miR1919c-5p and the accumulations of tobacco curly shoot virus (TbCSV) and its betasatellite (TbCSB) DNAs, as well as viral symptom development, we investigated the function of nbe-miR1919c-5p during TbCSV and TbCSB co-infection in plants using a PVX-and a TRV-based short tandem target mimic (STTM) technology. Suppression of nbe-miR1919c-5p expression using these two technologies enhanced TbCSV and TbCSB co-infection-induced leaf curling symptoms in plants. Furthermore, suppression of nbe-miR1919c-5p expression enhanced TbCSV and TbCSB DNA accumulations in the infected plants. Our results can advance our knowledge on the nbe-miR1919c-5p function during TbCSV and TbCSB co-infection.
MicroRNAs (miRNAs) 是长度为 21-25 个核苷酸的非编码但具有功能的 RNA 分子。miRNAs 在多种植物生物学过程中发挥重要的调节作用。为了解 nbe-miR1919c-5p 与烟草曲叶病毒（TbCSV）及其贝塔卫星（TbCSB）DNA 积累以及病毒症状发展之间的关系，我们使用 PVX 和 TRV 基于短串联靶标模拟（STTM）技术研究了 nbe-miR1919c-5p 在 TbCSV 和 TbCSB 共感染植物中的功能。使用这两种技术抑制 nbe-miR1919c-5p 的表达增强了植物中 TbCSV 和 TbCSB 共感染诱导的叶片卷曲症状。此外，抑制 nbe-miR1919c-5p 的表达增强了感染植物中 TbCSV 和 TbCSB DNA 的积累。我们的研究结果可以增进我们对 nbe-miR1919c-5p 在 TbCSV 和 TbCSB 共感染过程中的功能的认识。
Zotero 插件