College of Plant Protection, Southwest University, Chongqing, 400716, People's Republic of China.
Virol J. 2018 Sep 3;15(1):138. doi: 10.1186/s12985-018-1044-1.
Tobacco curly shoot virus (TbCSV) is a monopartite begomovirus associated with betasatellite (Tobacco curly shoot betasatellite, TbCSB), which causes serious leaf curl disease on tomato and tobacco in China. It is interesting that TbCSV induced severe upward leaf curling in Nicotiana benthamiana, but in the presence of TbCSB, symptoms changed to be downward leaf curling. However, the mechanism of interactions between viral pathogenicity, host defense, viral-betasatellite interactions and virus-host interactions remains unclear.
In this study, RNA-seq was used to analyze differentially expressed genes (DEGs) in N. benthamiana plants infected by TbCSV (Y35A) and TbCSV together with TbCSB (Y35AB) respectively.
Through mapping to N. benthamiana reference genome, 59,814 unigenes were identified. Transcriptome analysis revealed that a total of 4081 and 3196 DEGs were identified in Y35AB vs CK (control check) and Y35A vs CK, respectively. Both GO and KEGG analyses were conducted to classify the DEGs. Ten of the top 15 GO terms were enriched in both DEGs of Y35AB vs CK and Y35A vs CK, and these enriched GO terms mainly classified into three categories including biological process, cellular component and molecular function. KEGG pathway analysis indicated that 118 and 111 pathways were identified in Y35AB vs CK and Y35A vs CK, respectively, of which nine and six pathways were significantly enriched. Three major pathways in Y35AB vs CK involved in metabolic pathways, carbon metabolism and photosynthesis, while those in Y35A vs CK were related to Ribosome, Glyoxylate and dicarboxylate metabolism and DNA replication. We observed that 8 PR genes were significantly up-regulated and 44 LRR-RLK genes were significantly differentially expressed in Y35A treatment or in Y35AB treatment. In addition, 7 and 13 genes were identified to be significantly changed in biosynthesis and signal transduction pathway of brassinosteroid (BR) and jasmonic acid (JA) respectively.
These results presented here would be particularly useful to further elucidate the response of the host plant against virus infection.
烟草曲叶病毒（TbCSV）是一种与卫星 β（烟草曲叶卫星 β，TbCSB）相关的单分体伴生病毒，在中国引起番茄和烟草严重的卷叶病。有趣的是，TbCSV 在本氏烟上诱导严重的叶片上卷，但在存在 TbCSB 的情况下，症状变为叶片下卷。然而，病毒致病性、宿主防御、病毒-卫星相互作用和病毒-宿主相互作用之间的相互作用机制仍不清楚。
在这项研究中，使用 RNA-seq 分别分析了感染 TbCSV（Y35A）和 TbCSV 与 TbCSB（Y35AB）的本氏烟植株中的差异表达基因（DEGs）。
通过与本氏烟参考基因组进行比对，共鉴定出 59814 个 unigenes。转录组分析显示，Y35AB 与 CK（对照）和 Y35A 与 CK 相比，分别有 4081 和 3196 个 DEGs。GO 和 KEGG 分析均对 DEGs 进行了分类。Y35AB 与 CK 和 Y35A 与 CK 的前 15 个 GO 术语中有 10 个被富集，这些富集的 GO 术语主要分为生物过程、细胞成分和分子功能三个类别。KEGG 通路分析表明，Y35AB 与 CK 和 Y35A 与 CK 分别鉴定出 118 和 111 条通路，其中有 9 条和 6 条通路显著富集。Y35AB 与 CK 中三个主要通路涉及代谢途径、碳代谢和光合作用，而 Y35A 与 CK 中的通路则与核糖体、乙醛酸和二羧酸代谢以及 DNA 复制有关。我们观察到，Y35A 处理或 Y35AB 处理中 8 个 PR 基因显著上调，44 个 LRR-RLK 基因显著差异表达。此外，还分别鉴定到 7 个和 13 个基因在油菜素内酯（BR）和茉莉酸（JA）生物合成和信号转导途径中发生显著变化。
这些结果将有助于进一步阐明宿主植物对病毒感染的反应。
