Division of Applied Life Science (BK21 plus), Institute of Agriculture and Life Science Gyeongsang National University, Jinju 52828, Korea.
Nutrients. 2020 Mar 20;12(3):832. doi: 10.3390/nu12030832.
To evaluate possibility as a skin whitening agent of (), its antioxidant activity and anti-melanogenic effect on 3-isobutyl-1-methylxanthine (IBMX)-induced melanogenesis in B16/F10 melanoma cells were investigated. The result of total phenolic contents (TPC) indicated that 60% ethanol extract of (ESB) has the highest contents than other ethanol extracts. Antioxidant activity was evaluated using the 2,2'-azino-bis-(3-ethylbenzothiazolin-6-sulfonic acid) diammonium salt (ABTS)/1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activities and malondialdehyde (MDA) inhibitory effect. These results showed ESB has significant antioxidant activities. Inhibitory effect against tyrosinase was also assessed using L-tyrosine (IC value = 89.25 μg/mL) and 3,4-dihydroxy-L-phenylalanine (L-DOPA) as substrates. In addition, ESB treatment effectively inhibited melanin production in IBMX-induced B16/F10 melanoma cells. To confirm the mechanism on anti-melanogenic effect of ESB, we examined melanogenesis-related proteins. ESB downregulated melanogenesis by decreasing expression of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein (TRP)-1. Finally, 9-hydroxyoctadecadienoic acid (9-HODE), 1,3--dicaffeoylglycerol and tricin as the main compounds of ESB were analyzed using the ultra-performance liquid chromatography-ion mobility separation-quadrupole time of flight/tandem mass spectrometry (UPLC-IMS-QTOF/MS). These findings suggest that ESB may have physiological potential to be used skin whitening material.
为了评估（）作为皮肤美白剂的可能性，研究了其对 3-异丁基-1-甲基黄嘌呤（IBMX）诱导的 B16/F10 黑素瘤细胞中黑素生成的抗氧化活性和抗黑色素生成作用。总酚含量（TPC）的结果表明，（ESB）的 60%乙醇提取物比其他乙醇提取物具有更高的含量。抗氧化活性通过 2,2'-偶氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）/1,1-二苯基-2-苦基肼（DPPH）自由基清除活性和丙二醛（MDA）抑制作用来评估。这些结果表明 ESB 具有显著的抗氧化活性。还用 L-酪氨酸（IC 值=89.25μg/mL）和 3,4-二羟基-L-苯丙氨酸（L-DOPA）作为底物评估了对酪氨酸酶的抑制作用。此外，ESB 处理可有效抑制 IBMX 诱导的 B16/F10 黑素瘤细胞中的黑色素生成。为了证实 ESB 抗黑色素生成作用的机制，我们检查了与黑色素生成相关的蛋白质。ESB 通过降低小眼畸形相关转录因子（MITF）、酪氨酸酶和酪氨酸酶相关蛋白（TRP）-1 的表达来减少黑色素生成。最后，使用超高效液相色谱-离子迁移分离-四极杆飞行时间/串联质谱（UPLC-IMS-QTOF/MS）分析 ESB 的主要化合物 9-羟基十八碳二烯酸（9-HODE）、1,3--二咖啡酰甘油和 tricin。这些发现表明 ESB 可能具有作为皮肤美白材料的生理潜力。
