Lee Yuna, Song Ha-Yeon, Byun Eui-Baek
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea.
Heliyon. 2025 Jan 4;11(2):e41718. doi: 10.1016/j.heliyon.2025.e41718. eCollection 2025 Jan 30.
The suppression of tyrosinase (TYR), a key enzyme in melanogenesis, has been suggested as an effective strategy for preventing melanin accumulation. We previously discovered the novel chrysin derivative hydroxyethyl chrysin (HE-chrysin) through an irradiation technique, which exerted higher anti-inflammatory and anti-cancer activities than original chrysin. In the present study, we explored whether HE-chrysin has antioxidant and anti-melanogenic capacity using B16F10 murine melanoma cells and molecular docking. HE-chrysin exhibited enhanced antioxidant capacity in DPPH, ABTS, and FRAP assays, and it decreased cellular HO-stimulated reactive oxygen species levels in comparison to original chrysin. At 2.5 μM, HE-chrysin reduced 3-isobutyl-1-methylxanthine-stimulated melanin production significantly by suppressing intracellular TYR activity without cytotoxicity. Furthermore, molecular docking showed that HE-chrysin inhibited TYR activity by interacting with key residues (Glu and Asn) and chelating Cu ions at the active site, with a binding free energy of -7.00 kcal/mol compared with arbutin (-5.12 kcal/mol). Our findings show that HE-chrysin is an anti-melanogenic candidate and a potential antioxidant for use in dermatologic therapy.
抑制酪氨酸酶（TYR），一种黑色素生成中的关键酶，已被认为是预防黑色素积累的有效策略。我们之前通过辐照技术发现了新型白杨素衍生物羟乙基白杨素（HE-白杨素），其抗炎和抗癌活性高于原始白杨素。在本研究中，我们使用B16F10小鼠黑色素瘤细胞和分子对接技术探究了HE-白杨素是否具有抗氧化和抗黑色素生成能力。在DPPH、ABTS和FRAP测定中，HE-白杨素表现出增强的抗氧化能力，与原始白杨素相比，它降低了细胞内HO刺激的活性氧水平。在2.5 μM时，HE-白杨素通过抑制细胞内TYR活性显著降低了3-异丁基-1-甲基黄嘌呤刺激的黑色素生成，且无细胞毒性。此外，分子对接显示，HE-白杨素通过与关键残基（Glu和Asn）相互作用并在活性位点螯合铜离子来抑制TYR活性，与熊果苷（-5.12 kcal/mol）相比，其结合自由能为-7.00 kcal/mol。我们的研究结果表明，HE-白杨素是一种抗黑色素生成候选物，也是用于皮肤科治疗的潜在抗氧化剂。
