Department of Immunology and Institute of Medical Science, Jeonbuk National University Medical School, 20, Geonji-ro, Deokjin-gu, Jeonju-si 54907, Jeollabuk-do, Korea.
Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan 54538, Jeonbuk, Korea.
Molecules. 2021 Mar 1;26(5):1308. doi: 10.3390/molecules26051308.
When skin is exposed to UV radiation, melanocytes produce melanin. Excessive melanin production leads to skin pigmentation, which causes various cosmetic and health problems. Therefore, the development of safe, natural therapeutics that inhibit the production of melanin is necessary. (EU) has long been widely used as a folk medicinal plant because of pharmacological properties that include anti-ulcer, antioxidant, and anti-inflammatory properties. In this study, we investigated the antioxidant activity and melanogenesis inhibitory effects of EU fractions in B16-F10 melanoma cells. EU fractions showed a dose-dependent increase in antioxidant activity in radical scavenging activity. In addition, we evaluated the effect of EU fractions on tyrosinase activity and melanogenesis in α-melanocyte-stimulating hormone-induced B16-F10 melanoma cells. EU was noncytotoxic at 12.5-50 μg/mL. EU fractions effectively inhibited tyrosinase activity and melanogenesis, suppressed the phosphorylation of CREB and ERK involved in the melanogenesis pathway, and down-regulated expression of melanogenesis-related proteins. Interestingly, the anti-melanogenesis effect was most effective at a concentration of 50 μg/mL EU, and the effects of the fractions were superior to those of the extract. Therefore, our study suggests that EU has potential as a safe treatment for excessive pigmentation or as a natural ingredient in cosmetics.
当皮肤暴露在紫外线辐射下时，黑素细胞会产生黑色素。黑色素的过度产生会导致皮肤色素沉着，从而引发各种美容和健康问题。因此，开发安全、天然的抑制黑色素产生的疗法是必要的。
欧洲菘蓝（EU）因其具有抗溃疡、抗氧化和抗炎等药理作用，长期以来一直被广泛用作民间药用植物。在本研究中，我们研究了 EU 馏分在 B16-F10 黑色素瘤细胞中的抗氧化活性和黑色素生成抑制作用。EU 馏分在自由基清除活性方面表现出剂量依赖性的抗氧化活性增加。此外，我们评估了 EU 馏分对 α-促黑素细胞激素诱导的 B16-F10 黑色素瘤细胞中酪氨酸酶活性和黑色素生成的影响。EU 在 12.5-50 μg/mL 时无细胞毒性。EU 馏分能有效抑制酪氨酸酶活性和黑色素生成，抑制黑色素生成途径中涉及的 CREB 和 ERK 的磷酸化，并下调黑色素生成相关蛋白的表达。有趣的是，EU 浓度为 50 μg/mL 时对黑色素生成的抑制作用最为显著，且馏分的效果优于提取物。因此，我们的研究表明，EU 具有作为治疗过度色素沉着的安全疗法或作为化妆品中天然成分的潜力。
