灌注鸡肾中的糖异生作用。进食与饥饿的影响。

Gluconeogenesis in perfused chicken kidney. Effects of feeding and starvation.

Yamano T, Yorita K, Fujii H, Uchimoto R, Shiota M, Ohta M, Sugano T

Department of Veterinary Physiology, College of Agriculture, University of Osaka Prefecture, Japan.

Comp Biochem Physiol B. 1988;91(4):701-6. doi: 10.1016/0305-0491(88)90195-2.

Abstract

Starvation for 48 hr doubled the rate of gluconeogenesis from lactate and pyruvate in perfused chicken kidney, but did not change the rate of production of glucose from malate, succinate, or alpha-ketoglutarate. 2. Amino-oxyacetate and D-malate inhibited the production of glucose from lactate and from pyruvate by 55% in each case. Quinolinate reduced the production of glucose from lactate and from pyruvate by 50% in both fed and starved chickens, but had no effect on the production of glucose from intermediates in the citric acid cycle. 3. Starvation increased the rate of formation of mitochondrial phosphoenolpyruvate from pyruvate, but had no effect on the rate of formation of mitochondrial phosphoenolpyruvate from malate.

摘要

饥饿48小时使灌注鸡肾中由乳酸和丙酮酸生成葡萄糖异生作用的速率加倍，但不改变由苹果酸、琥珀酸或α-酮戊二酸生成葡萄糖的速率。2. 氨基氧乙酸和D-苹果酸分别使由乳酸和丙酮酸生成葡萄糖的量减少55%。喹啉酸在喂食和饥饿的鸡中均使由乳酸和丙酮酸生成葡萄糖的量减少50%，但对柠檬酸循环中间产物生成葡萄糖的过程没有影响。3. 饥饿增加了由丙酮酸生成线粒体磷酸烯醇式丙酮酸的速率，但对由苹果酸生成线粒体磷酸烯醇式丙酮酸的速率没有影响。