Basimi Parya, Akbari Asbagh Firouzeh, Yekaninejad Mir Saeed, Asadi Mojgan, Dabbagh Ali, Najafi Arbastan Farangis, Rajaei Samira
Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Obstetrics and Gynecology, Yas Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2020 Feb 1;19(1):65-73. doi: 10.18502/ijaai.v19i1.2419.
Thyroid autoimmunity, being recognized by the presence of auto-antibodies against thyroid peroxidase (TPO) and thyroglobulin, has known to be associated with increased risk of recurrent spontaneous abortion (RSA), even in euthyroid subjects. There was no robust evidence regarding T cell deviations in anti-TPO positive RSA patients. The aim of this study was to investigate if the numbers of different CD4+T subsets were different in women who experienced RSA and have an anti-TPO antibody from those without autoantibody and normal fertile women or not. In this study, peripheral blood samples were obtained from three groups of women (age: 20-35 years) including RSA anti-TPO positive (n=17), RSA anti-TPO negative (n=27), and fertile (n=29) groups. The frequency of T helper (Th) 1, Th2, Th17, and regulatory T cells (Tregs) and also, the proportions of Th1/Th2 and Th17/Treg were measured by flow cytometry and compared between groups in different menstrual phases. The findings indicated elevated levels of Th1 in anti-TPO+ RSA in comparison with those without anti-TPO (p-value: 0.004), exclusively in the luteal phase. Other T cell subsets were different only between RSA and control groups. Also, the Th1/Th2 and Th17/Treg ratios were increased in both RSA groups compared to fertile women. The only subset of CD4+ T cell different between RSA groups (i.e. with and without anti-TPO) was Th1 cells. Other CD4+ T cells' deviations including Th2, Th17, and Treg cells could be related to the presence of abortion, regardless of the underlying thyroid autoimmunity state.
甲状腺自身免疫是通过抗甲状腺过氧化物酶（TPO）和甲状腺球蛋白自身抗体的存在来识别的，已知其与复发性自然流产（RSA）风险增加有关，即使在甲状腺功能正常的受试者中也是如此。关于抗TPO阳性RSA患者的T细胞偏差，尚无确凿证据。本研究的目的是调查有抗TPO抗体的RSA女性与无自身抗体的正常育龄女性相比，不同CD4 + T亚群的数量是否存在差异。在本研究中，从三组年龄在20 - 35岁的女性中采集外周血样本，包括RSA抗TPO阳性组（n = 17）、RSA抗TPO阴性组（n = 27）和育龄组（n = 29）。通过流式细胞术测量辅助性T细胞（Th）1、Th2、Th17和调节性T细胞（Tregs）的频率，以及Th1/Th2和Th17/Treg的比例，并在不同月经周期的组间进行比较。研究结果表明，与无抗TPO的女性相比，抗TPO + RSA女性的Th1水平升高（p值：0.004），且仅在黄体期。其他T细胞亚群仅在RSA组和对照组之间存在差异。此外，与育龄女性相比，两个RSA组的Th1/Th2和Th17/Treg比值均升高。RSA组（即有和无抗TPO）之间唯一不同的CD4 + T细胞亚群是Th1细胞。其他CD4 + T细胞偏差，包括Th2、Th17和Treg细胞，可能与流产的发生有关，而与潜在的甲状腺自身免疫状态无关。
