Department of Pharmacology, School of Medicine, Southeast University, Nanjing 210009, China.
Chin J Nat Med. 2020 Mar;18(3):169-177. doi: 10.1016/S1875-5364(20)30018-2.
The objective of this study was to verify the protective effect of Bifidobacterium longum (BL) and the synergistical effect of Selenium and BL on alcohol plus high fat diet (HFD) induced hepatic injury in mice. We also want to explore the mechanism of Selenium-enriched Bifidobacterium longum (SeBL). C57BL/6 mice were treated with alcohol plus HFD with or without different dosage of BL or SeBL for 4 weeks. Serum levels of ALT, AST, TC, TG, LDL-C, HDL-C, FFAs, TNF-α, IL-6 and IL-1β, hepatic MDA level, SOD activity, the mRNA levels of AMPK, PPAR-α and SREBP1 were invested. SeBL inhibited lipid accumulation in hepatocytes; reduced serum AST and ALT levels; improved dyslipidemia; decreased serum FFAs, TC, TG and LDL-C levels. SeBL also inhibited alcohol plus HFD-induced hepatocyte oxidative stress through decrease in hepatic MDA levels and increase in SOD activity. SeBL also regulated lipid metabolism related genes such as AMPK, PPAR-α and SREBP1. Although BL had similar effect as SeBL, SeBL is more effective than BL. SeBL protected mice from alcohol plus HFD-induced hepatic injury in mice because of its inhibitory effect on hepatocellular oxidative stress, lipogenesis and inflammation. Selenium enhanced the protective effect of BL.
本研究旨在验证长双歧杆菌（BL）的保护作用以及硒与 BL 对酒精加高脂饮食（HFD）诱导的小鼠肝损伤的协同作用。我们还想探讨富硒长双歧杆菌（SeBL）的作用机制。用酒精加 HFD 处理 C57BL/6 小鼠 4 周，同时给予不同剂量的 BL 或 SeBL。检测血清 ALT、AST、TC、TG、LDL-C、HDL-C、FFAs、TNF-α、IL-6 和 IL-1β、肝 MDA 水平、SOD 活性、AMPK、PPAR-α 和 SREBP1 的 mRNA 水平。SeBL 抑制肝细胞脂质堆积；降低血清 AST 和 ALT 水平；改善血脂异常；降低血清 FFAs、TC、TG 和 LDL-C 水平。SeBL 通过降低肝 MDA 水平和增加 SOD 活性，抑制酒精加 HFD 诱导的肝细胞氧化应激。SeBL 还调节 AMPK、PPAR-α 和 SREBP1 等脂质代谢相关基因。虽然 BL 具有与 SeBL 相似的作用，但 SeBL 比 BL 更有效。SeBL 抑制酒精加 HFD 诱导的小鼠肝损伤，因为它抑制了肝细胞氧化应激、脂肪生成和炎症。硒增强了 BL 的保护作用。
