Department of Chemistry, Durham University, South Road, Durham, DH1 3LE, UK.
Nat Commun. 2020 Apr 3;11(1):1676. doi: 10.1038/s41467-020-15469-5.
Circular polarisation luminescence (CPL) emission spectroscopy is a powerful tool for probing the fundamental chiroptical features of optically emissive chiral molecular systems. However, uptake of CPL spectroscopy has been impeded by the limitations of conventional scanning monochromator (SM) CPL spectrometers, which are costly to acquire and maintain, and typically require tens of minutes to acquire a typical CPL spectrum. Here, we demonstrate a design of CPL spectrometer which uses rapid readout solid state (SS) spectrometer detectors and a dual channel optical layout to acquire CPL spectra in as little as 10 milliseconds. We validate and demonstrate equivalent CPL measurement by measuring CPL spectra of two reference europium(III) complexes. Further, we demonstrate time-gated CPL acquisition, enabling long-lived CPL luminescence to be distinguished from short-lived emission of other fluorescent species. We anticipate that SS-CPL spectrometers will enable flexible, rapid, and relatively low-cost CPL spectroscopy for diverse applications.
圆偏振发光（CPL）发射光谱学是探测光学发光手性分子体系基本手性光学特性的有力工具。然而，传统扫描单色仪（SM）CPL 光谱仪的局限性阻碍了 CPL 光谱学的应用，这些光谱仪购置和维护成本高，通常需要数十分钟才能获得典型的 CPL 光谱。在这里，我们展示了一种 CPL 光谱仪的设计，该设计使用快速读取固态（SS）光谱仪探测器和双通道光学布局，在短短 10 毫秒内获取 CPL 光谱。我们通过测量两个参考铕（III）配合物的 CPL 光谱来验证和演示等效的 CPL 测量。此外，我们还演示了时间门控 CPL 采集，能够将长寿命 CPL 发光与其他荧光物质的短寿命发射区分开来。我们预计 SS-CPL 光谱仪将为各种应用提供灵活、快速且相对低成本的 CPL 光谱学。
