Department of Agricultural Bioscience, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa, Japan.
Sci Rep. 2020 Apr 3;10(1):6039. doi: 10.1038/s41598-020-58942-3.
Our understanding regarding the influence of intensive agricultural practices, including cover cropping and tillage, on communities of arbuscular mycorrhizal fungi (AMF) is lacking. This would prove to be an obstacle in the improvement of current maize (Zea mays L.) production. Therefore, using amplicon sequencing, we aimed to clarify how AMF communities and their diversity in maize roots vary under different cover cropping systems and two types of tillage (rotary and no tillage). Two kinds of cover crops (hairy vetch and brown mustard) and fallow treatments were established with rotary or no tillage in rotation with maize crops. Tillage and no tillage yielded a set of relatively common AMF operational taxonomic units (OTUs) in the maize crops, representing 78.3% of the total OTUs. The percentage of maize crop OTUs that were specific to only tillage and no tillage were 9.6% and 12.0%, respectively. We found that tillage system significantly altered the AMF communities in maize roots. However, the AMF communities of maize crops among cover cropping treatments did not vary considerably. Our findings indicate that compared with cover cropping, tillage may shape AMF communities in maize more strongly.
我们对于包括覆盖作物和耕作在内的集约化农业实践对丛枝菌根真菌（AMF）群落的影响的了解还很缺乏。这将成为当前玉米（Zea mays L.）生产改进的障碍。因此，我们使用扩增子测序来阐明在不同的覆盖作物系统和两种耕作方式（旋耕和免耕）下，玉米根系中的 AMF 群落及其多样性是如何变化的。在与玉米轮作的情况下，采用旋耕或免耕建立了两种覆盖作物（毛叶苕子和芥菜）和休耕处理。耕作和免耕在玉米作物中产生了一组相对常见的 AMF 操作分类单元（OTUs），占总 OTUs 的 78.3%。仅耕作和免耕特有的玉米作物 OTUs 分别占 9.6%和 12.0%。我们发现耕作系统显著改变了玉米根系中的 AMF 群落。然而，覆盖作物处理下的玉米作物 AMF 群落变化不大。我们的研究结果表明，与覆盖作物相比，耕作可能更强烈地影响玉米中的 AMF 群落。
