Department of Chemistry, Vanderbilt University, Nashville, TN, USA.
Neuroscience Program, Vanderbilt University, Nashville, TN, USA.
Methods Mol Biol. 2020;2135:109-123. doi: 10.1007/978-1-0716-0463-2_6.
Single quantum dot tracking (SQDT) is a powerful technique for interrogating biomolecular dynamics in living cells and tissue. SQDT has particularly excelled in driving discovery at the single-molecule level in the fields of neuronal communication, plasma membrane organization, viral infection, and immune system response. Here, we briefly characterize various elements of the SQDT analytical framework and provide the reader with a detailed set of executable commands to implement commonly used algorithms for SQDT data processing.
单量子点追踪 (SQDT) 是一种强大的技术，可用于研究活细胞和组织中的生物分子动力学。在神经元通讯、质膜组织、病毒感染和免疫系统反应等领域，SQDT 尤其擅长在单分子水平上推动发现。在这里，我们简要描述了 SQDT 分析框架的各个要素，并为读者提供了一组详细的可执行命令，用于实现 SQDT 数据处理常用算法。
