Yumoto T, Naniwa S, Yoshida H, Nahiro J, Kasagi N, Maeda N
Department of Pathology, Tottori University School of Medicine, Japan.
Exp Pathol. 1988;35(3):177-87. doi: 10.1016/s0232-1513(88)80145-2.
Gardner's murine osteosarcoma has been successfully cultured and maintained as a clonal cell line (GOS/T), capable of forming a tumor with neoplastic bone and osteoid tissue by inoculation of 1 x 10(7) cultured cells. Alkaline phosphatase (ALPase) level in the culture medium and the serum from the tumor-bearing mice increased significantly three times and 16 to 105 times, respectively. The bone-specific ALPase was purified by butanol extraction following gel-filtration through Sephadex G-200. The molecular weight of ALPase was determined as 420,000, which was three times 140,000, the molecular weight of a subunit. Immunofluorescence staining using anti-ALPase antiserum, which was made by inoculation of partially purified ALPase, revealed a positive reaction for GOS/T cell line and osteoblasts of the fetal mice. The data presented here suggest that the cell line of GOS/T can be regarded as an established cell line, and the immunological reaction with anti-ALPase antibody is a useful model system to study human osteosarcoma.
加德纳氏小鼠骨肉瘤已成功培养并维持为克隆细胞系（GOS/T），通过接种1×10⁷个培养细胞能够形成含有肿瘤性骨和类骨组织的肿瘤。培养基和荷瘤小鼠血清中的碱性磷酸酶（ALPase）水平分别显著升高了3倍和16至105倍。骨特异性ALPase经葡聚糖凝胶G - 200凝胶过滤后用丁醇提取法纯化。测定ALPase的分子量为420,000，是亚基分子量140,000的3倍。使用通过接种部分纯化的ALPase制备的抗ALPase抗血清进行免疫荧光染色，结果显示GOS/T细胞系和胎鼠成骨细胞呈阳性反应。此处呈现的数据表明，GOS/T细胞系可被视为一种已建立的细胞系，并且与抗ALPase抗体的免疫反应是研究人类骨肉瘤的有用模型系统。
